结肠癌组织miR-200c／141定量表达的临床病理学意义  

Clinicopathological significance of quantitative expression of mi R-200c /141 in colon carcinoma tissue

作　　者：孟令宽[1] 钟小莉[1] 潘琼[1] 叶钧[1] 尚杨杨[1] 郭靖[1] 汪荣泉[1] 

机构地区：[1]重庆第三军医大学西南医院消化内科,400038

出　　处：《解放军医学杂志》2015年第12期987-992,共6页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金(81372557)~~

摘　　要：目的探讨结肠癌组织mi R-200c/141定量表达与患者临床病理学特征之间的联系。方法实时定量PCR检测49例结肠癌患者癌组织和癌旁组织中的mi R-200c/141的表达水平。分析mi R-200c/141表达量、联合mi R-200c和mi R-141表达量与患者临床病理学特征之间的关系,以及mi R-200c/141表达量在判断患者术后1年预后中的意义。结果结肠癌组织中mi R-200c/141表达水平较癌旁组织明显降低(P=0.0002,P=0.0058)。mi R-141表达量与肿瘤大小和淋巴结转移呈负相关(P<0.05),有淋巴结转移或临床Ⅲ-Ⅳ期患者结肠癌组织中mi R-141的表达量明显低于没有淋巴结转移或临床Ⅰ-Ⅱ期患者癌组织的表达量(P=0.035,P=0.042)。mi R-200c/141表达量与肿瘤大小之间明显相关(P<0.05)。mi R-200c/141的表达量与患者术后1年的生存率无相关关系(P>0.05)。结论结肠癌组织中表达下调的mi R-200c/141与肿瘤大小、淋巴结转移、临床分期有关。Objective To explore the relationship between the miR-200c and miR-141 expression levels in the cancerous tissues of colon carcinoma and the clinicopathological features of the patients. Methods The quantitative real-time RT-PCR was used to determine the miR-200c and miR-141 expression levels in the cancerous tissues obtained from 49 colon carcinoma patients and their tissues adjacent to the cancer. Results Compared with the tissues adjacent to the cancer, the miR-200c and miR-141 expression levels in colon carcinoma samples were significantly reduced （P=0.0002 and P=0.0058）. A negative correlation was found between miR-141 expression level and both the tumor size and the existence of lymph node metastasis in the colon carcinoma patients （P〈0.05）. The miR-141 expression levels in the cancerous tissues of colon carcinoma patients who had lymph node metastasis were significantly lower than those who did not have lymph node metastasis （P=0.035）. At the same time, the miR-141 expression levels in the cancerous tissues of colon carcinoma patients who were at clinicalⅢ -Ⅳ stages were significantly lower than in those who were at clinical Ⅰ - Ⅱ stages （P=0.042）. There was a significant correlation between the miR-200c/141 expression levels and tumor size （P=0.021）. However, no relationship was found between the miR-200c and miR-141 expression levels and the cumulative survival rate of patients one-year after surgical treatment （P〉0.05）. Conclusion The down-regulation ofmiR-200c/141expression in colon carcinoma tissues is related to the tumor size, lymph node metastasis and clinical stages of colon carcinoma patients.

关 键 词：结肠肿瘤 病理学 临床 微RNAS 

分 类 号：R574.62[医药卫生—消化系统]