小鼠脑出血后脑葡萄糖代谢变化的18 F-FDG micro PET／CT显像研究  

Changes of glucose metabolism in mouse models of intracerebral hemorrhage：18 F-FDG microPET/CT study

作　　者：杨凡慧 王琴[1] 张春银[1] 谭华[2] 杨朝鲜[3] 王威[3] 尤强[1] 

机构地区：[1]泸州医学院附属医院核医学科,646000 [2]泸州医学院附属医院神经内科,646000 [3]泸州医学院神经生物学研究室

出　　处：《中华核医学与分子影像杂志》2015年第6期483-486,共4页Chinese Journal of Nuclear Medicine and Molecular Imaging

基　　金：四川省科学技术厅与泸州市人民政府、泸州医学院联合科研专项资金计划项目（14JC0131）

摘　　要：目的：利用18 F-FDG microPET／CT显像技术，探讨小鼠脑出血（ ICH）后脑葡萄糖代谢变化的规律。方法选取12只普通级健康雄性昆明小鼠，单纯随机抽样法分为假手术对照组（ A组）和ICH模型组（ B组），每组6只。用Ⅳ型胶原酶制备ICH模型。分别于建模前及建模后6、24、48 h及3、5、8、14 d共8个时间点麻醉小鼠后，经尾静脉注入18 F-FDG 5．5 MBq，30 min后行microPET／CT显像，并测定出血灶部位的SUV。组间SUV比较采用两样本t检验。结果（1） A组小鼠仅部分在假手术后早期存在轻度神经功能缺损，B组小鼠有明显的神经精神症状，24 h时最为明显。（2） micro.PET／CT显像示，B组小鼠在血肿形成6 h 后，血肿周围脑组织的18 F-FDG 摄取减低（ SUV＝0．80±0.04），低于镜像侧（SUV＝1．10±0．04），差异有统计学意义（t＝2．69，P＜0．05）；术后24 h摄取达最低水平（SUV＝0．50±0．05），3 d后开始恢复（SUV＝1．20±0．05），14 d后进一步恢复。 B组小鼠脑出血灶各时间点的葡萄糖代谢均低于A组小鼠，差异有统计学意义（ t＝37．67～86．60，均P＜0．05）。结论18 F-FDG microPET／CT能动态反映ICH小鼠的脑葡萄糖代谢变化；随着时间的推移，血肿葡萄糖代谢减低区域逐渐缩小，中心为葡萄糖代谢缺失区。Objective To investigate the brain glucose metabolism with 18 F-FDG microPET/CT in mouse models of intracerebral hemorrhage （ICH）. Methods A total of 12 healthy adult male mice were randomly divided into sham operation group （group A, n=6） and ICH model group （group B, n=6） by simple random sampling method. The animal models were established by injecting collagenase Ⅳ into the caudate nucleus of mice. Thereafter （5.5±0.3） MBq of 18F-FDG was injected into caudal vein at 6 h, 24 h, 48 h and 3 d, 5 d, 8 d, 14 d, respectively, following anesthesia. 18 F-FDG microPET/CT scans were ac.quired 30 min after the trace injection. SUV in the perihematomal brain tissue of ICH was measured and an.alyzed. Two.sample t test was used to compare SUV between groups. Results （ 1） Some mice had mild neurologic deficit after the sham operation in group A, while all mice had a marked neurologic deficit in group B, especially at 24 h after 18 F-FDG injection. （ 2） After 6 h, FDG uptake in perihematomal brain tis.sue decreased（SUV=0.80±0.04）, which significantly lower than that in the opposite side（SUV=1.10± 0.04;t=2.69, P〈0.05） and decreased to the minimum at 24 h（SUV=0.50±0.05）. 18F-FDG uptake in perihematomal brain tissue began to increase at 3 d（SUV=1.20±0.05） and kept increasing during the 14 d observation. Compared with the group A, glucose metabolism in group B was significantly lower at each time point（t=37.67-86.60, all P〈0.05）. Conclusions 18 F-FDG microPET/CT may dynamically reflect the changes of brain glucose metabolism in ICH mouse models. The FDG uptake in the center of ICH may disap.pear and the volume of hematoma with decreased uptake may shrink during the observation period.

关 键 词：脑出血 体层摄影术 发射型计算机 体层摄影术 X线计算机 脱氧葡萄糖 小鼠 

分 类 号：R743.34[医药卫生—神经病学与精神病学] R817.1[医药卫生—临床医学]