高效液相色谱法测定艾附暖宫丸中芍药苷和阿魏酸的含量  

Simultaneous determination of peoniflorin and ferulic acid in Aifunuangong Pills by HPLC method

作　　者：李洪亮[1] 乔静[1,2] 

机构地区：[1]湖北医药学院附属人民医院中药药理实验室,湖北十堰442000 [2]湖北医药学院药学院,湖北十堰442000

出　　处：《中国医药导报》2015年第36期21-23,31,共4页China Medical Herald

基　　金：湖北省教育厅指导项目(B2014046);湖北省十堰市科技局引导性项目(15Y36)

摘　　要：目的 建立高效液相色谱法测定艾附暖宫丸中芍药苷、阿魏酸的含量。方法 采用Sinochrom ODS-BP（4.6 mm×250 mm,5μm）色谱柱,流动相为乙腈︰0.03 mol/L NH4Ac（含0.25%甲酸）（13︰87）,流速：0.9 m L/min,检测波长：230-321 nm,柱温：30℃。结果 芍药苷在5.2-41.6μg/m L范围内线性关系良好,r=0.998 32（n=5）,平均回收率为100.5%,RSD=1.07%（n=9）;阿魏酸在0.489-3.916μg/m L范围内线性关系良好,r=0.999 716（n=5）,平均回收率为96.8%,RSD=1.05%（n=9）,4批样品中芍药苷平均含量分别为1.41、1.38、1.51、1.72 mg/L,阿魏酸平均含量为0.90、0.86、0.60、0.74 mg/g。结论 本法简便﹑准确﹑灵敏度高,重现性好,可作为艾附暖宫丸的含量测定。Objective To establith a novel method for simultaneous determination of peoniflorin and ferulic acid in Ai- funuangong Pills by HPLC method. Methods Sinochrom ODS-BP column （4.6 mmx250 mm, 5 μm） was used, with acetonitrile： 0.03 mol/L ammonium acetate （including 0.25% methanoic acid） （13 ： 87） as mobile phase. The flow rate was 0.9 mL/min with the detection wavelength at 232-312 nm. The column temperature was 30℃. Results The linear range of peoniflorin was from 5.2 to 41.6 Ixg/mL （r = 0.998）. The average recovery was 100.5% （RSD = 1.07%）. The linear range of ferulic acid was from 0.489 to 3.916 μg/mL （r = 0.9997）. The average recovery was 96.8% （RSD = 1.05%）, in the 4 grups,the average content of peoniflorin was 1.41, 1.38, 1.51, 1.72 mg/g, ferulic acid was 0.90, 0.86, 0.60, 0.74 mg/g. Conclusion The method J s simple, accurate, sensitive and reproducible and may be potential criterion for the quality control of Aifunuangong ？ills.

关 键 词：艾附暖宫丸 芍药苷 阿魏酸 高效液相色谱 

分 类 号：R927.2[医药卫生—药学]