长链非编码RNA BC088414在神经细胞缺氧缺血损伤中的作用  

Role of long non-coding RNA BC088414 in hypoxic-ischemic injury of neural cells

作　　者：赵凤艳[1] 唐军[1] 张莉[1] 李世平[1] 冯艺[1] 刘海婷[1] 屈艺[1] 母得志[1] 

机构地区：[1]四川大学华西第二医院儿科,妇儿疾病与出生缺血教育部重点实验室,四川成都610041

出　　处：《中国当代儿科杂志》2015年第12期1348-1353,共6页Chinese Journal of Contemporary Pediatrics

基　　金：国家自然科学基金(81300526;81300524);教育部博士点基金(20120181120049);国家临床重点专科(儿科新生儿专业)建设项目(1311200003303)

摘　　要：目的探讨长链非编码RNA BC088414在神经细胞缺氧缺血损伤中的作用。方法将大鼠肾上腺嗜铬细胞瘤(PC12)细胞分为常氧组、氧糖剥夺处理组(OGD组)、小分子RNA常氧组(si RNA组)及si RNA OGD组,n=3。以无葡萄糖无血清DMEM培养液于37℃、1%O2+99%N2/CO2条件下培养细胞6 h,建立缺氧缺血体外模型。采用实时定量PCR法检测BC088414、肾上腺素受体β2(Adrb2)和半胱氨酸蛋白酶6(Casp6)m RNA的表达;利用小分子干扰RNA(si RNA)抑制PC12细胞中BC088414的表达;采用TUNEL法检测细胞凋亡情况。结果 OGD组细胞凋亡指数明显高于常氧组(P<0.01),抑制BC088414表达后,PC12细胞的凋亡指数明显低于OGD组(P<0.05)。经OGD处理后,PC12细胞中BC088414、Adrb2和Casp6 m RNA表达水平较常氧组均明显增加(P<0.05);抑制BC088414表达,可导致PC12细胞中Adrb2和Casp6 m RNA表达水平较常氧组降低,且可抑制OGD诱导的Adrb2和Casp6 m RNA表达上调(P<0.05)。结论长链非编码RNA BC088414可能通过Adrb2和Casp6促进凋亡,加重缺氧缺血引起的神经细胞损伤。Objective To investigate the role of long non-coding RNA（lnc RNA） BC088414 in hypoxic-ischemic injury of neural cells. Methods Rat adrenal pheochromocytoma（PC12） cells were divided into four groups： normoxic, oxygen glucose deprivation（OGD）, si RNA-normoxic（si RNA group） and si RNA-OGD（n=3 each）. Cells were incubated in glucose-free and serum-free DMEM medium under the conditions of 37°C and 1% O2＋99% N2/CO2 for 6 hours to establish an in vitro hypoxic-ischemic model. Quantitative real-time PCR was used to measure m RNA expression of lnc RNA BC088414, β2-adrenoceptor（Adrb2）, and caspase-6（CASP6）. si RNAs were used to inhibit BC088414 expression in PC12 cells. The TUNEL method was used to measure cell apoptosis. Results The OGD group had a significantly higher cell apoptotic index than the normoxic group（P〈0.01）. After inhibition of BC088414 expression, the OGD group had a significantly reduced apoptotic index（P〈0.05）. The OGD group had significantly higher m RNA expression levels of lnc RNA BC088414, Adrb2, and CASP6 compared with the normoxic group（P〈0.05）. The si RNA-normoxic group had significantly lower m RNA expression levels of Adrb2 and CASP6 than the normoxic group（P〈0.05）, and the si RNA-OGD group also had significantly lower m RNA expression levels of Adrb2 and CASP6 than the OGD group（P〈0.05）. Conclusions Lnc RNA BC088414 may promote apoptosis through Adrb2 and CASP6 and aggravate neural cell injury induced by hypoxia-ischemia.

关 键 词：长链非编码RNA 氧糖剥夺 肾上腺嗜铬细胞瘤细胞 

分 类 号：R742[医药卫生—神经病学与精神病学]