支链氨基酸氨基转移酶1在恶性胶质瘤中的表达及意义  

Expression and significance of branched-chain amino acid transaminase 1 in malignant gliomas

作　　者：张国滨[1] 张晋[2] 徐恒周 周益强[2] 程森[2] 刘福生[2] 

机构地区：[1]首都医科大学附属北京天坛医院神经外科,100050 [2]首都医科大学北京市神经外科研究所

出　　处：《中华神经外科杂志》2015年第12期1259-1262,共4页Chinese Journal of Neurosurgery

基　　金：基金项目：国家自然科学基金（81372354）;北京市卫生系统高层次卫生技术人才培养计划（2013-2-018）;科研基地建设-北京实验室-生物医用材料北京实验室[京教涵（2013）133号];首都医科大学附属北京天坛医院青年科研课题（2013-YQN-12）

摘　　要：目的 探讨不同级别神经胶质瘤中支链氨基酸氨基转移酶1（BCAT1）的表达水平及其与肿瘤增殖的关系.方法 收集首都医科大学附属北京天坛医院神经外科2012至2013年手术切除的54例神经胶质瘤标本,免疫组化检测54例神经胶质瘤中BCAT1和Ki-67的表达水平;实时荧光定量PCR检测BCAT1基因的表达程度.结果 （1）BCAT1表达水平随着病理分级的增高而增多（P＜0.05）;（2）其表达程度与Ki-67表达呈正相关（r=0.825,P＜0.01）;（3）随着胶质瘤恶性程度的增高,BCAT1 mRNA的表达增高,WHO Ⅰ级（0.5587±0.2038）和Ⅱ级（1.3499±0.3964）之间的表达差异具有统计学意义（P＜0.01）,WHOⅡ级和Ⅲ级之间的表达差异具有统计学意义（P＜0.01）,WHOⅢ级（2.0004±0.4025）和Ⅳ级（3.0656±0.4955）之间的表达差异具有统计学意义（P＜0.01）.结论 BCAT1与神经胶质瘤恶性程度呈正相关,恶性程度越高其BCAT1表达水平越高.Objective To investigate the expression levels of branched-chain amino acid transaminase 1 （BCAT1） and its relationship with glioma proliferation in different levels of glioma.Methods The glioma specimens of 54 patients resected at Department of Neurosurgery,Beijing Tiantan Hospital,Capital Medical University from 2012 to 2013 were collected.Immunohistochemistry assay was used to detect the expression levels of BCAT1 and Ki-67 in 54 patients with glioma.Real-time fluorescence quantitative PCR was used to detect the expression degree of BCAT1 gene.Results （1） The expression levels of BCAT1 increased with the elevated pathologic grades （P 〈 0.05）.（2） Its expression degree is positively correlated with that of Ki-67 （r =0.825,P 〈 0.01）.（3） With the increase of the malignant degree of gliomas,the expression of BCAT1 mRNA increased.There was significant difference in the expression levels between the WHO grade Ⅰ （0.5587 ± 0.2038） and grade Ⅱ （1.3499 ± 0.3964） （P 〈 0.01）.There was significant difference in the expression levels between the WHO grade Ⅱ and grade Ⅲ （P 〈0.01）.There was significant difference in the expression levels between the WHO grade Ⅲ （2.0004 ±0.4025） and grade Ⅳ （3.0656±0.4955） （P〈0.01）.Conclusions BCAT1 is positively correlated with the malignant degree of gliomas.The higher the degree of malignancy,the higher the expression level of BCAT1.

关 键 词：神经胶质瘤 支链氨基酸氨基转移酶1 细胞增殖 

分 类 号：R739.41[医药卫生—肿瘤]