恶性肿瘤患者外周血淋巴细胞免疫表型检测的临床意义  

Clinical significance of detecting peripheral blood lymphocyte immunophenotype in patients with malignant carcinoma

作　　者：章梁君 钟锦莎 钟辉秀 刘伟平[1] 殷明刚 杨新春[1] 

机构地区：[1]自贡市第一人民医院检验科,四川自贡643000 [2]成都中医药大学,四川成都610072

出　　处：《国际检验医学杂志》2015年第24期3565-3567,共3页International Journal of Laboratory Medicine

摘　　要：目的研究恶性肿瘤患者外周血淋巴细胞免疫表型变化及其临床意义。方法采用流式细胞术检测167例恶性肿瘤患者组(包括呼吸道癌、胃肠道癌、生殖系统癌)外周血中T淋巴细胞亚群、CD19^+B细胞、CD3-CD56^+自然杀伤细胞(NK)细胞、CD3^+CD56^+自然杀伤T细胞(NKT)细胞免疫表达水平,设170例健康对照组进行对比分析,并根据有无肿瘤转移和化疗周期进行分析。结果恶性肿瘤患者总淋巴细胞绝对值,CD3^+CD4^+比例明显低于健康对照组(P<0.05),而CD3^+CD8^+、CD3^+CD56^+、CD3-CD56^+比例却明显高于健康对照组(P<0.05)。肿瘤转移患者组CD3^+CD4^+比例明显低于未发生肿瘤转移患者组(P=0.011),而CD3^+CD8^+、CD3-CD56^+、CD3^+CD56^+比例明显高于未发生肿瘤转移患者组,差异均有统计学意义(P<0.05)。在化疗治疗中,CD3^+CD4^+、CD3^+CD56^+、CD3-CD56^+比例随着化疗周期增加而逐渐增高,而CD3^+CD8^+比例呈逐渐降低。结论在肿瘤的发生、发展和治疗中,细胞免疫功能起主导地位,可作为临床监测肿瘤患者细胞免疫功能状态的指标之一。Objective To investigate the change of peripheral blood lymphocyte immunophenotype in the patients with malignant carcinoma and its clinical significance.Methods The flow cytometry was adopted to detect the expression levels of peripheral blood T lymphocyte subpopulations,CD19~＋cells,CD3-CD56~＋cells,CD3~＋CD56~＋cells in 167 patients with malignant carcinoma（including respiratory tract cancers,gastrointestinal cancers and reproductive system cancer）and 170 normal controls as the healthy control group.The contrastive analysis was performed.Furthermore the analysis was conducted according to whether having tumor metastasis and chemotherapeutic cycles.Results The total absolute value of the lymphocytes and the proportion of CD3~＋CD4~＋cells in the patients with malignant carcinoma were significantly lower than those in the healthy control group（P〈0.05）,but the proportions of CD3~＋CD8~＋,CD3~＋CD56~＋and CD3-CD56~＋cells were significantly higher than those in the healthy control group（P〈0.05）.The proportion of CD3~＋CD4~＋cells in the patients with tumor metastasis were significantly lower than those in the ones without tumor metastasis;but the proportions of CD3~＋CD8~＋,CD3-CD56~＋and CD3~＋CD56~＋cells were significantly higher than those in the ones without tumor metastasis,the differences were statistically significant（P〈0.05）.In the chemotherapy treatment,the proportions of CD3~＋CD4~＋,CD3~＋CD56~＋and CD3-CD56~＋cells were gradually increased with the increase of chemotherapeutic cycles,but the proportion of CD3~＋CD8~＋cells was gradually reduced.Conclusion In the process of development,progression and treatment of tumor,the cellular immunity function plays a dominant role,which could be used as one of indexes for clinically monitoring the cellular immune function in the patients with tumor.

关 键 词：辅助性T淋巴细胞 细胞毒性T淋巴细胞 自然杀伤细胞 自然杀伤T细胞 

分 类 号：R730.43[医药卫生—肿瘤]