玉竹根际土壤化感作用研究及化感物质的鉴定  

Study on Allelopathy and Identification of Allelochemical in Rhizosphere Soil of Polygonatum

作　　者：肖岚[1] 刘应蛟 徐贝[1] 喻亚飞 刘塔斯[1] 

机构地区：[1]湖南中医药大学药学院,长沙410208

出　　处：《中国农学通报》2015年第34期163-167,共5页Chinese Agricultural Science Bulletin

基　　金：国家科技部支撑课题"玉竹;茯苓规范化种植及其SOP优化升级研究"(2011BAI101B09)

摘　　要：通过对玉竹根际土壤提取液的生物学试验和有机化合物的分离鉴定,探讨玉竹连作障碍与化感作用的关系。采用浸提法制备不同浓度的玉竹根际土壤浸提液,用室内生物测定法测定玉竹的化感效应;采用气相色谱-质谱联用技术(GC-MS)鉴定玉竹根际土壤有机化合物。结果表明:玉竹根际土壤浸提液能显著抑制玉竹自身根茎芽胞发芽率、幼苗苗高及根鲜重,且随浸提液浓度的升高,这种抑制作用增强。在玉竹根际土壤浸提液中鉴定到24个化合物,包括有机酸、酮、醛等化感物质,其中很多被报道是化感物质。可得出结论:玉竹根际土壤浸提液对自身根茎芽胞和幼苗生长具有明显的化感作用,且自毒效应具有一定的浓度依赖性,推断化感作用可能是造成玉竹连作障碍的原因之一。The relationship of continuous cropping obstacle and allelopathy of Polygonatum was studiedthrough the biology tests of soil extracting solution and the isolation and identification of organic compounds.Extracting method was used to prepare rhizosphere soil extracting solution of Polygonatum, indoor biologicalassay method was used to measure allelopathy and GC-MS was used to identify the organic compounds. Theresults showed that the rhizosphere soil extracting solution of Polygonatum could significantly inhibit therhizome spore germination rate, seedling height and root fresh weight, and with the increase of extractionsolution concentration, the inhibition was enhanced. 24 kinds of compounds were identified in the extractingsolution, including organic acids, ketones, aldehydes and so on, many of which had been reported to beallelochemicals. Rhizosphere soil extracting solution of Polygonatum had obvious allelopathy on rhizome sporegermination rate and seedling growth, and the self-poison effect had dependency on concentration, indicatingthat allelopathy was the reason that caused continuous cropping obstacle of Polygonatum.

关 键 词：玉竹 根际土壤 化感物质 连作障碍 气相色谱-质谱联用技术 

分 类 号：S36[农业科学—作物栽培与耕作技术]