苹果腐烂病菌侵染过程中枝条相关病程物质含量的变化  

Change of Disease Related Substances Content in Branches During the Process of Valsa ceratosperma

作　　者：刘斐[1] 谭贻[1] 王忆[1] 吴婷[1] 张新忠[1] 

机构地区：[1]中国农业大学农学与生物技术学院果树系,北京100193

出　　处：《中国农学通报》2015年第34期177-186,共10页Chinese Agricultural Science Bulletin

基　　金：公益性行业(农业)科研专项"我国重要野生果树资源的收集;评价与优异种质创新利用技术研究与示范"(201303093);国家863课题"苹果分子育种与种质创制"(2011AA001204)

摘　　要：为探究苹果腐烂病的抗病机制,分别用中抗腐烂病种质‘法8’和‘高5’,感病种质‘紫云’和‘红富士’以及高感种质‘嘎拉’和‘金冠’的1年生休眠枝离体接种腐烂病菌株03-8的菌丝,接种后0~7天检测接种部位周围皮层健康组织中病程相关物质多酚、水杨酸和木质素含量变化。结果表明：所有种质接种后1~7天皮层中对香豆酸、肉桂酸和绿原酸含量均显著升高,但仅绿原酸含量与腐烂病的病斑长度显著负相关（r=-0.906）。接菌后皮层组织水杨酸和木质素含量显著增加,水杨酸和木质素含量与病斑长度显著负相关（r=-0.825;r=-0.948）。因此,接种苹果腐烂病菌后皮层水杨酸、木质素和绿原酸含量的显著增加与苹果抗腐烂病相关。To elucidate the physiological mechanism of the resistance to apple canker, one-year-old applebranches of the moderately resistant accessions‘French 8＇and‘Gao 5＇, the susceptible cultivars‘Whitney＇and‘Red Fuji＇and highly susceptible cultivars‘Gala＇and‘Golden delicious＇ were inoculated in vitro withmycelia of Valsa ceratosperma isolate 03- 8. Zero to seven days after the inoculation, the changes inpolyphenols, salicylic acid and lignin were detected. The results indicated that 0- 7 days after inoculation,p- coumaric acid, cinnamic acid and chlorogenic acid increased significantly, but only the content ofchlorogenic acid was negatively correlated with Valsa canker lesion length（r=-0.906）. After inoculation withmycelia, salicylic acid and lignin contents in the cortical tissue significantly increased, the content of salicylicacid and lignin was significantly and negatively correlated with Valsa canker lesion length（r=- 0.825;r=-0.948）. Therefore, the increase of salicylic acid, lignin and chlorogenic acid in apple was correlated withthe resistance to Valsa ceratosperma.

关 键 词：苹果腐烂病 抗病性 木质素 多酚 水杨酸 

分 类 号：S661.1[农业科学—果树学]