塔里木盆地17个引进红枣品种HPLC指纹图谱及定量测定的研究  

HPLC fingerprint and quantitative determination of 17 introduced Ziziphus jujube varieties in Tarim Basin

作　　者：刘丹[1] 隋月红[1] 陈朋[1] 沈立荣[2] 白红进[1] 

机构地区：[1]塔里木大学生命科学学院新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆维吾尔自治区阿拉尔843300 [2]浙江大学馥莉食品研究院,浙江杭州310058

出　　处：《中草药》2015年第21期3248-3252,共5页Chinese Traditional and Herbal Drugs

基　　金：兵团科技局农社处科技援疆专项资助(2013AB023);浙江大学馥莉食品研究院基金资助项目(KY201404);塔里木大学研究生科研创新项目(TDGRI201511)

摘　　要：目的建立塔里木盆地17个引进红枣品种高效液相色谱指纹图谱方法,为红枣品质的科学评价和产品的质量控制提供科学依据。方法采用HPLC法,色谱柱TOSOH TSKgel ODS-100V C18柱(150 mm×4.6 mm,3μm),流动相为甲醇-0.3%磷酸二氢钾缓冲水溶液,梯度洗脱,体积流量1.0 m L/min,柱温40℃,检测波长260 nm。对采自塔里木大学种质资源圃中17个引进品种的红枣进行指纹图谱研究,用药典委员会颁布的中药色谱指纹图谱相似度评价系统2004A进行评价。结果建立了红枣的指纹图谱,标定了22个共有峰,各峰分离度良好,并对尿嘧啶、次黄嘌呤、腺嘌呤、胞苷、尿苷、环磷酸腺苷(c AMP)、环磷酸鸟苷(c GMP)进行测定。结论 HPLC指纹图谱分析技术可用于红枣的鉴别。Objective To initially establish HPLC fingerprints for analysis of 17 batches of the introdued jujube（Ziziphus jujuba） varieties in order to lay the foundation for scientific evaluation and production quality control of jujube. Methods TOSOH TSKgel ODS-100 V C18 column（150 mm × 4.6 mm, 3 μm） was used. The mobile phase was as follows： methanol and 0.3% KH2PO4 in gradient elution mode. The detective wavelength was 260 nm, the flow rate was 1.0 m L/min, and the separation was performed at 40 ℃. Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2004 A published by the state Pharmacopeia Committee of China was adopted for the fingerprint analysis on the 17 batches of introdued jujube varieties in Germplasm Nursery of Tarim University. Results The HPLC characteristic fingerprint of jujube has been established. A total of 22 common peaks were characterized, the peaks appeared uniform and were well-separated. The uracil, hypoxanthine, adenine, cytidine, uridine, c AMP, and c GMP were determined by HPLC method. Conclusion HPLC chromatographic fingerprint analysis technology can be used for the identification of jujube and its quality control.

关 键 词：红枣 高效液相色谱 指纹图谱 尿嘧啶 次黄嘌呤 腺嘌呤 胞苷 尿苷 c AMP c GMP 

分 类 号：R286.022[医药卫生—中药学]