真菌聚酮合酶基因研究进展  

Scientific advances in fungal polyketide synthase genes

作　　者：孙书荃 董章勇[1] 张云霞[1] 向梅梅[1] 

机构地区：[1]仲恺农业工程学院农学院,广东广州510225

出　　处：《仲恺农业工程学院学报》2015年第4期66-70,共5页Journal of Zhongkai University of Agriculture and Engineering

基　　金：国家自然科学基金(30671382)资助项目

摘　　要：真菌聚酮化合物是一类重要的次生代谢产物,其结构和功能多样,生物学活性广泛.聚酮合酶(Polyketide synthase,PKS)是催化聚酮化合物合成的关键酶.近几年,随着基因组测序技术、分子生物学技术和遗传分析工具、表达宿主、重组酶制剂等的发展,真菌聚酮合酶基因的研究取得了许多新的进展.文章对近年来在真菌聚酮合酶基因获得、基因功能验证及真菌聚酮合酶基因工程方面的研究进展进行了综述,并对该领域的研究热点进行了展望.Fungal polyketides are very important secondary metabolites with structural and functional diversities and wide-ranging bioactivities. Polyketide synthase（ PKS） is the key enzyme that catalyzes the biosynthesis of polyketide. In recent years,some scientific advances in PKS genes had been achieved along with the developments in genome sequencing and molecule biological technology as well as tools of genetic analysis,heterologous express hosts,recombination enzyme preparations etc. In this paper,the progresses in the discovery and functional verification of PKS genes and genetic engineering of PKS were reviewed,and the research focuses in this area were prospected.

关 键 词：真菌聚酮化合物 聚酮合酶 基因发掘 生物合成 

分 类 号：Q933[生物学—微生物学] Q36