养血清脑颗粒对血管性痴呆大鼠海马CA1区胶质纤维酸性蛋白表达的影响  

Effect of Yangxue Qingnao Granule on Expression of Glial Fibrillary Acidic Protein in CA1 Area of Hippocampus in Rats with Vascular Dementia

作　　者：董静 李晶 刘斌 毛文静 张晋霞 李世英 

机构地区：[1]华北理工大学附属医院康复科,河北唐山市063000 [2]华北理工大学附属医院神经内一科,河北唐山市063000

出　　处：《中国康复理论与实践》2015年第12期1375-1378,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：河北省高等学校科学技术研究重点项目(No.ZH2012046);河北省级重大医学科研课题(No.zd2013087)

摘　　要：目的观察养血清脑颗粒对血管性痴呆大鼠海马CA1区胶质纤维酸性蛋白(GFAP)表达的影响。方法 144只Sprague-Dawley大鼠随机分为假手术组(n=48)、血管性痴呆模型组(模型组,n=48)和养血清脑颗粒治疗组(治疗组,n=48)。采用改良Pulsinelli四血管阻断法制作血管性痴呆大鼠模型。假手术组和模型组灌胃生理盐水10 ml/(kg?d);治疗组灌胃养血清脑颗粒3.2g/(kg?d)。分别在术后1周、2周、4周和8周采用免疫组化法和Western blotting法检测海马CA1区GFAP表达水平。结果与对照组比较,模型组各时间点大鼠海马CA1区GFAP表达明显增高(P<0.01);与模型组比较,治疗组各时间点GFAP表达明显下降(P<0.01)。结论养血清脑颗粒可抑制血管性痴呆大鼠海马CA1区星形胶质细胞的增生和活化。Objective To investigate the effect of Yangxue Qingnao granule on the expression of glial fibrillary acidic protein（GFAP） in CA1 area of hippocampus in rats with vascular dementia（VD）. Methods 144 Sprague-Dawley rats were randomly divided into sham operation group（n=48）, VD group（model group, n=48） and Yangxue Qingnao granule treatment group（treatment group, n=48）. The VD model was prepared by modified Pulsineli＇s four-vessel occlusion. The sham operation group and the model group were given normal saline 10 ml/（kg · d） by gavage, and the treatment group was given Yangxue Qingnao granule 3.2 g/（kg · d） by gavage. The expression of GFAP in CA1 area of hippocampus was detected with immunohistochemical analysis and Western blotting method 1, 2, 4 and 8 weeks after modeling. Results The expression of GFAP in CA1 area of hippocampus was significantly higher in the model group than in the sham operation group（P〈0.01）, and was lower in the treatment group than in the model group（P〈0.05） at every time point. Conclusion Yanxue Qingnao granule could inhibit the activation and proliferation of astrocytes in rats.

关 键 词：血管性痴呆 星形胶质细胞 胶质纤维酸性蛋白 养血清脑颗粒 大鼠 

分 类 号：R749.1[医药卫生—神经病学与精神病学]