百蕊草物候期及越冬休眠生理研究  

Study on winter dormancy of Thesium chinense and its phenological phase

作　　者：宋玲珊[1] 张晓明[1] 郭巧生[1] 陈璐[1] 王长林[1] 

机构地区：[1]南京农业大学中药材研究所,江苏南京210095

出　　处：《中国中药杂志》2015年第23期4585-4590,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81173482)

摘　　要：为探索百蕊草人工栽培模式,以茅山地区百蕊草为实验材料,对百蕊草物候期及其越冬休眠过程碳水化合物变化规律进行研究。采用定点标记法观察百蕊草物候期,硫酸蒽酮法测定可溶性糖和淀粉含量,DNS法测定淀粉酶活力,PAS-鉄钒苏木精双重染色组织定位淀粉粒。在连续3年的观察中未发现可存活2年以上百蕊草植株,可推断百蕊草在长江流域属于二年生植物;第1年为实生苗,生长周期较长。出苗较早的实生苗在夏季倒苗死亡,而出苗较晚的可顺利越夏并在秋冬季分化出根颈芽,冬季休眠。第2年为宿根苗,由根颈芽发育成簇生状,生长周期较短;根具储藏根的结构与功能特征,休眠期储藏淀粉等碳水化合物,储藏物为翌年快速生长发育的能量基础。根内淀粉和可溶性糖在不同时期相互转化,使百蕊草在休眠过程中既有充足的碳水化合物来维持生长也有丰富的小分子溶质来提高其抗逆能力,提高其生长能力及顺利越冬。In order to explore reasonable artificial cultivation pattern of Thesium chinense,the biological characteristics and nutrients change in the process of winter dormancy of T. chinense was studied. The phenological period of T. chinense was observed by using fixed-point notation and the starch grains changes were determined dynamically by PAS-vanadium iron hematoxylin staining method.Soluble sugar and starch content were measured by anthrone-sulfuric acid method and amylase activity was determined by DNS method.The results showed that the normal life cycle of T. chinense was two years. T. chinense was growing by seed in the first year,but growing by the root neck bud in the second year. During the process of dormancy,starch and soluble sugar could mutual transformation in different periods. T. chinense had sufficient carbohydrate to maintain growth and also a lot of small molecules to improve their ability to fight against adversity.

关 键 词：百蕊草 物候期 休眠 碳水化合物 

分 类 号：S567.239[农业科学—中草药栽培]