树舌多糖对人胃癌SGC-7901细胞增殖及CyclinD1、p16表达的影响  

Effect of ganoderma applanatum polysaccharides on human gastric carcinoma Cell SGC-7901,anti-proliferation and exprssion of CyclinD1,p16

作　　者：吉爱军[1] 陆建伟[2] 李苏平[3] 井昶雯[3] 

机构地区：[1]江苏省肿瘤医院镇痛科,江苏南京210009 [2]江苏省肿瘤医院肿瘤内科 [3]江苏省肿瘤医院科研科

出　　处：《中国肿瘤外科杂志》2015年第6期356-359,共4页Chinese Journal of Surgical Oncology

基　　金：江苏省中医药局科技项目(项目编号:LZ13231)

摘　　要：目的探讨树舌多糖对人胃癌SGC-7901细胞增殖及CyclinD1、p16表达的影响,进一步研究其抗胃癌的可能机制。方法培养人胃癌SGC-7901细胞,MTT法观察不同时间(24 h,72 h)不同浓度树舌多糖对人胃癌SGC-7901细胞增殖的影响。用流式细胞法检测树舌多糖对细胞周期阻滞及其凋亡的影响。实时荧光定量法检测树舌多糖作用人胃癌SGC-7901细胞72 h后CyclinD1及p16的表达情况。结果随着树舌多糖浓度增高和作用时间的延长,对人胃癌SGC-7901细胞增殖抑制率逐渐增高(P<0.05),同一浓度不同时间的细胞增殖抑制率相比有显著性差异(P<0.05)。人胃癌SGC-7901细胞在G0/G1期表现出明显的增加,S期细胞明显的减少,各浓度组均明显诱导细胞凋亡,其凋亡率随树舌多糖浓度增加而提高。实时荧光定量法检测显示,随着树舌多糖浓度的增加,CyclinD1表达逐渐减少,p16表达逐渐增加。树舌多糖浓度0.5mg/ml,1.0 mg/ml时,CyclinD1及p16表达存在显著性差异(P<0.05)。结论树舌多糖对人胃癌SGC-7901细胞有抑制增殖和促凋亡作用,呈时间和浓度依赖性,且G0/G1期细胞数量增多,S期细胞数量减少。RT-PCR法检测显示,CyclinD1呈现低表达,p16呈现高表达,由此推测,CyclinD1表达下调与p16表达上调可分别发挥细胞周期的正、负反馈调节,共同作用产生细胞增殖抑制及促进凋亡,从而发挥抗肿瘤作用。Objective To investigate the effects of Ganoderma applanatum polysaccharides （GAPS） on pro- liferation and apoptosis of the human gastric carcinoma cell line 7901 and its possible mechanism of action on CyclinD1 ,p16. Methods The growth inhibition effect of GAPS on human gastric carcinoma cell line 7901 was observed by using MTT assay, and cell apoptosis was analyzed by flow cytometry. The expression of cy- clinDlmRNA and p16mRNA in SGC-7901 cells were analyzed by real-time fluorescence quantitative PCR. Re- suits The SGC-7901 cells were treated with various concentrations of GAPS for 48 h and 72 h, cell viability was determined by Alamar Blue assay. Cell growth was suppressed by GAPS of different Concentrations （0,0. 125 mg/ml,0.25 mg/ml, 0.5 mg/ml, 1.0 mg/ml） , GAPS inhibited the growth of gastric cancer cells in a dose-dependent manner. The cells were treated with various concentrations （0,0.25 mg/ml, 0.5 mg/ml, 1.0 mg/ml） of GAPS for 72 h. The results showed that GAPS were capable of inducing an increase in the percenta- ges of Gl-phase cells and the number of apoptotic cells （P 〈 0.05 ）. Real-time quantitative PCR was used to detect the mRNA expression of CyclinD1 and p16 at 72 h after GAPS treatment. The results revealed a signifi- cant down-regulation of mRNA expression of CyclinD1, and up-regulation of the mRNA expression levels of p16 as compared with the controls. Conclusions The results show that GAPS has significant action of anti-prolifera- tion and pro-apoptosis on human gastric carcinoma cell line 7901. The down-regulation of CyclinD1 suppresses the cell proliferation inhibition and promotes the cell apoptosis, p16 showed high expression, which can activate the negative feedback of cell cycle. The results suggest that GAPS may have an effect on the pathway of gastric cancer genesis,it prevents the cells from G1/G0 phase to S phase.

关 键 词：树舌多糖 胃癌 SGC-7901 增殖 CYCLIN D1 p16 

分 类 号：R285[医药卫生—中药学]