浦东地区部分畜禽养殖场沙门氏菌的分离鉴定  

Identification of Salmonella in Livestock and Poultry Farms from the Pudong Area

作　　者：韩少鹏[1] 俞向前[2] 魏强 胡龙明 叶承荣[2] 朱建国[1,2] 

机构地区：[1]上海交通大学农业与生物学院上海市兽医生物重点实验室,上海200240 [2]上海市浦东新区动物疫病预防控制中心,上海201299 [3]上海魏强畜禽养殖场,上海201322 [4]浦东新区农业服务中心新场站,上海201314

出　　处：《上海交通大学学报（农业科学版）》2015年第6期36-40,共5页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：浦东新区科技发展基金创新资金(PKJ2013-N18和PKJ2012-N01);上海市生猪产业体系建设项目资助

摘　　要：为了检测上海市浦东地区部分养殖场沙门氏菌阳性率,建立了沙门氏菌flim、invA、hns及hut基因四重PCR检测方法,对2014年03月至08月期间来自浦东地区患沙门氏菌的100份病、死猪禽内脏器官进行细菌分离、革兰氏染色、四重PCR鉴定及分离株flim基因序列分析。结果显示,浦东地区部分猪禽场沙门氏菌阳性率为23%（23/100）;分离株flim基因序列与GenBank上已公布的沙门氏菌flim基因核苷酸同源性为98%~100%;进化树图谱表明,分离株flim基因与副伤寒沙门氏菌、肠炎沙门氏菌及猪霍乱沙门氏菌亲缘性相近。In order to detect the positive rate of Salmonellain part of livestock and poultry farms in Pudong area of Shanghai,a multiplex（4）PCR detection of Salmonella was established.From Mar.to Aug.2014,sick or dead pigs or poultries affected with salmonellosis were sampled.The 100 strains of bacteria,isolated from 100 internal organs,were tested by gram stain and multiple PCR method,and the flim gene of isolates were analyzed.The results showed that the positive rate of Salmonellain part of livestock and poultry farms in Pudong area were 23%.Compared flimgene sequence of isolates with those of Salmonella published on GenBank,the identification homology of flim gene is 98%-100%,and the isolates of Salmonella show similar affinity with Salmonella paratyphi,Salmonella enteritidis and Salmonella cholerasuis in the phylogenetic tree.

关 键 词：沙门氏菌 多重PCR 检测方法 同源性 

分 类 号：S852.611[农业科学—基础兽医学]