基于气液双相-质谱联用技术对轻微型肝性脑病患者血清代谢物谱的分析  

Research on serum metabolite spectrum of patients with minimal hepatic encephalopathy

作　　者：王萌[1] 黄国初[1] 姚春[2] 张宜男 钟宇[2] 杨小徽[2] 

机构地区：[1]广西中医药大学第一附属医院脾胃病科,广西南宁530023 [2]广西中医药大学 [3]上海交通大学第六人民医院

出　　处：《中西医结合肝病杂志》2015年第6期328-330,381,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：国家自然科学基金项目(No.81160439)

摘　　要：目的:通过对肝硬化、轻微型肝性脑病(MHE)患者血清代谢物的分析,找出MHE特异性代谢物谱。方法:采用气相色谱-质谱技术(GC-TOFMS)和液相色谱-质谱技术(UPLC-QTOFMS)研究23例肝硬化失代偿期患者(肝硬化组)、29例MHE患者(MHE组)和50例健康人(对照组,NC)血清中的代谢物,应用主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)对代谢物谱数据进行模式识别分析。结果:用代谢组学方法鉴定了29个差异代谢物,其中21个代谢物上调,包括各种次级胆汁酸、胆酸盐、酪氨酸、山梨醇、神经酸、柠檬酸、肌酐等,以胆汁酸、胆酸盐增高最为显著。下调代谢物包括支链氨基酸、草酸、神经鞘氨醇、甲状腺素、维生素E、牛磺酸。结论:胆汁酸、胆酸盐的代谢紊乱影响脂质代谢,加重肝细胞毒作用;芳香族氨基酸/支链氨基酸比例失调、神经鞘氨醇含量下降是MHE患者发生认知功能改变的原因之一。Objective：To seek out the specific metabolite profiling of cirrhosis patients with minimal hepatic encephalopathy. Methods： serum metabolites of 23 cases of liver cirrhosis patients（cirrhosis group）, 29 cases of patients with minimal hepatic en-cephalopathy（MHE group）and 50 healthy people（control group） were detected by using GC-TOFMS and UPLC-QTOFMS, the outcome raw data were analyzed by using principal component analysis（PCA） and orthogonal partial least squares- discriminant analysis（OPLS-DA） for pattern recognition analysis. Results： Twenty-nine different metabolites were identified, among them, 21 were increased, including a variety of secondary bile acids, cholate, tyrosine, sorbitol, nerve acid, citric acid, creatinine, etc., bile acids and bile salts increased the most; 8 were decreased, including branched-chain amino acids, oxalic acid, sphingosine, thyroxine, vitamin E and taurine. Conclusion： Metabolic disorders of the bile acids and bile salts affect the lipid metabolism and increase the hepatic cytotoxicity. Imbalance of aromatic amino acids and branched-chain amino acid ratio and sphingosine decreasing might be the reasons for the cognitive function disorders of MHE.

关 键 词：轻微型肝性脑病 气相色谱-质谱技术 液相色谱-质谱技术 代谢组学 

分 类 号：R575.3[医药卫生—消化系统]