高脂性脂肪肝小鼠模型的建立  

Establishment of hyperlipidemic fatty liver model in BALB/c Mice

作　　者：马方 王宁 陈慧平 王庆端 邹敏 张艳 江金花 

机构地区：[1]河南省医药科学研究院药化研究室,河南郑州450052

出　　处：《中西医结合肝病杂志》2015年第6期347-348,I0009,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：河南省自然科学基金研究项目(No.152300410160);河南省自然科学基金研究项目(No.142300410398)

摘　　要：目的:探讨建立高脂性脂肪肝小鼠模型的方法。方法:BALB/c小鼠腹腔注射地塞米松和灌服高脂乳剂,检测小鼠血脂及肝脂水平,观察肝脏组织病理学改变情况,探讨高脂性脂肪肝小鼠模型的建立。结果:与正常对照组相比,脂肪肝模型组小鼠血清中甘油三脂(TG)、总胆固醇(TC)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)均显著性升高(<0.01);肝匀浆中TG、TC含量及肝脏系数亦显著性增高(<0.01);病理检查可见其肝组织脂肪变性及炎性浸润,成功建立高脂性脂肪肝小鼠模型。结论:通过腹腔注射地塞米松和灌服高脂乳剂可较快建立BALB/c小鼠高脂性脂肪肝模型。Objective：To study and establish the animal model of hyperlipidemic fatty liver in BALB/c mice. Methods： Hyperlipidemic fatty liver model（HFLM） was produced by injecting dexamethasone sodium phosphate injection in the BALB/c mice＇abdomen and feeding mice high-fat diet at one time. The TC, TG, ALT, AST in the serum and the TC, TG in the liver were measured. Liver tissues were observed with optical microscope. Results： Compared with the normal control group, the ALT, AST, TG and TC were all increased significantly, the level of TG and TC in liver homogenate and liver index were increased significantly, and hepatic steatosis and inflammatory infiltration were observed by pathological examination in the HFLM group. Conclusion： BALB/c mice HFLM were successfully established by injecting DEX plus high fat diet.

关 键 词：动物模型 地塞米松 高脂性脂肪肝 BALB/C小鼠 

分 类 号：R575.5[医药卫生—消化系统] R-332[医药卫生—内科学]