XPF基因多态性与膀胱癌发病风险的关联分析  

Study on the Association of XPF Polymorphisms and Risk of Bladder Cancer

作　　者：陈宇[1] 李菡[1] 林夏雯[1] 韩勍[2] 

机构地区：[1]南京大学医学院附属鼓楼医院核医学科,江苏南京210008 [2]南京医科大学附属南京儿童医院检验科,江苏南京210008

出　　处：《标记免疫分析与临床》2015年第12期1208-1213,共6页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨XPF基因的3个多态性位点（rs28360317、rsl805377、rs2836007）的基因型与膀胱癌及其病理参数的相关性。方法采用病例对照研究，使用Taqman检测技术对270例膀胱癌患者和270名正常对照的3个多态性位点的基因型分布进行分析。采用logistic回归模型分析各基因型与膀胱癌的发生及其病理参数的关系。结果XPF基因的3个多态性位点在病例及对照组中分布差异均没有统计学意义（P〉0．05）。rs2836007的CT基因型（CTvs．CC：OR=2．30，95％CI：1．33-3．99）、1Tr基因型（TrVS．CC：OR=3．50，95％CI：1．14～10．77）在高分化膀胱癌组与正常对照组中的分布频率差异有统计学意义（P〈0．05）。但rs2836007位点各基因型在肿瘤分期、周围淋巴结转移、远端淋巴结转移、低分化组、中分化组中的分布频率与对照组相比差异均无统计学意义（P〉0．05）。rs28360317和rsl805377位点与膀胱癌病理参数无显著相关性（P〉0．05）。结论本研究发现XPF基因的rs28360317、rs1805377、rs2836007位点与膀胱癌的发病不相关，rs2836007与高分化膀胱癌相关。Objective To investigate the relationship between the polymorphisms in XPF （rs28360317, rs1805377, rs2836007 ） and risk of bladder cancer and its clinieopathological parameters. Methods A case- control study was carried out based on 270 patients with bladder cancer and 270 health controls. The genotypes of the variants were detected by Taqman SNP Genotyping Assays. Logistic regression was applied to assess the association of the polymorphisms and the risk and pathologic characteristics of bladder cancer. Results There was no significant difference of genotype distribution of rs28360317, rs1805377 and rs2836007 between the bladder cancer and control groups （ P 〉 0.05 ）. The CT genotype of rs2836007 was associated with well- differentiated bladder cancer （ adjusted odds ratio （ OR）, 2.30 ; 95 % confidence interval （ CI ）, 1.33- 3.99 ）, and TT genotype of rs2836007 was also associated with well-differentiated bladder cancer（ adjusted OR, 3.50; 95% CI, 1. 14-10. 77） compared with the AA genotype of rs2836007 in patients and healthy controls. However, none of the genotypes of rs2836007 exhibited any significant differences between the tumor differentiation degree, poorly differentiated bladder cancer, differentiated bladder cancer and healthy controls respectively. Results showed that there was no significant difference between either rs28360317 or rs1805377 with pathologic characteristics of tumor of bladder cancer and control groups in our study population （P 〉 0. 05 ）. Conclusion These findings suggest that the three polymorphisms（ rs28360317, rs1805377, rs2836007 ） were not associated with the risk of bladder cancer; Moreover, rs2836007 was associated with well- differentiated bladder cancer.

关 键 词：膀胱癌 XPF 基因多态性 

分 类 号：R737.14[医药卫生—肿瘤]