靶向PDGFR-α mRNA的RNA干扰抑制实验性增生性玻璃体视网膜病变  

Inhibition of targeted platelet-derived growth factor receptor-α RNA interference into vitreous cavity for experimental proliferative vitreoretinopathy

作　　者：孟竹[1] 彭燕一[2] 秦贤杰[1] 

机构地区：[1]桂林医学院,中国广西壮族自治区桂林市541004 [2]桂林医学院附属医院,中国广西壮族自治区桂林市541001

出　　处：《国际眼科杂志》2016年第1期24-27,共4页International Eye Science

基　　金：广西自然科学基金项目(No.2012GXNSFAA053103);广西医学科学实验中心开放课题项目(No.KFJJ2011-11)~~

摘　　要：目的:观察血小板源性生长因子-α受体(platelet-derived growth factor receptor,PDGFR-α)mRNA的RNA干扰抑制实验性增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)的效果。方法:选择PDGFR-αshRNA/lip2000比值1:1、1:2、1:3(其中PDGFR-αshRNA分别为2μg、3μg和4μg)制备复合物转染至人视网膜色素上皮细胞(human retinal pigment epithelium,HRPE)中,24h后分别取0.1mL注射到家兔玻璃体腔。选取健康成年有色家兔40只随机分为平衡盐溶液(balanced salt solution,BSS)组(N组),含lipofeetamine^(TM)2000的HRPE细胞稀释液组(A组),取最佳转染效率的1.0、1.5与2.0μmol/L含PDGF-α受体的shRNA及lipofeetamine^(TM)2000的HRPE细胞稀释液组(B、C和D组),每组8眼,右眼为实验眼。间接眼底镜观察PVR程度,免疫组织化学染色进行切片着色情况观察及组织病理学观察眼底改变。结果:PDGFR-αshRNA/lip2000比值1:2时,HRPE细胞最佳转染效率;B组、C组和D组PVR程度、组织病理学改变、免疫组织化学染色PDGFR-α浓度低于A组,D组比B组和C组更低。结论:PDGF-α受体mRNA的RNA干扰对实验性PVR形成有抑制作用。AIM：To observe the therapeutic effect of platelet-derived growth factor receptor-α RNA interference on inhibiting experimental proliferative vitreoretinopathy（PVR）. METHODS：Different concentrations of PDGFR-α shRNA were blended with lip2000,and the final scale of PDGFR-α shRNA/lip2 000 complex was 1：1, 1：2 and 1：3 respectively（the concentration of PDGFR-α shRNA was respectively 2μg, 3μg and 4μg）.All the complexes were cultivated in human retinal pigment epithelium（HRPE）for 24h after transfection and then respectively injected 0.1mL to rabbit vitreous cavity.Forty healthy adult rabbits were seleceted in this study and randomly divided into colored balanced salt solution（BSS）group（N）, comprising lipofectamineTM2 000 HRPE cell dilution group（group A）. The highest transduction efficiency of 1.0, 1.5 and 2.0μmol/L containing PDGFR-α receptors shRNA, lipofectamineTM2 000 of HRPE cell dilution were selected（respectively group B, C and D）with 8 eyes each, the right eyes as the experimental eye. The extent of PVR was observed by indirect ophthalmoscope; and slice staining situation was observed by immunohistochemistry.The fundus changes were observed by histopathology. RESULTS：The highest transduction efficiency of PDGFR-αshRNA/lip2 000 ratio was 1：2.The extent of PVR, the histopathology changes and the immunohistochemistry of PDGFR-α in group B,C and group D were significantly lower than that in group A, while group D was much lower than those of group B and C. CONCLUSION：PDGFR-αRNA interference could inhibit the formation of experimental PVR.

关 键 词：视网膜色素上皮细胞 增生性玻璃体视网膜病变 PDGF-α受体 RNA干扰 

分 类 号：R774.1[医药卫生—眼科]