木瓜蛋白酶提取蜚蠊多糖最佳工艺研究  

Study on the Best Extraction Process of Polysaccharide from Periplaneta americana by Papain

作　　者：李晓庆[1] 富尧[1] 陈光道 韩晓玥[1] 常江春 罗志文[1] 许龙[1] 

机构地区：[1]佳木斯大学生命科学学院,黑龙江佳木斯154007

出　　处：《安徽农业科学》2015年第34期81-82,110,共3页Journal of Anhui Agricultural Sciences

基　　金：佳木斯大学研究生科技创新项目(LZZ2014_002)

摘　　要：[目的]优化木瓜蛋白酶提取美洲大蠊多糖的工艺参数。[方法]利用不同梯度木瓜蛋白酶对美洲大蠊进行蜚蠊多糖提取试验,采用单因素和正交试验的方法,考察最佳酶用量、提取时间、温度等相关参数对提取液中多糖的含量的影响,确定蜚蠊多糖提取的工艺。[结果]试验表明,蜚蠊多糖提取的最佳条件为:酶用量为300μg/g,提取温度60℃,提取时间1.0 h。[结论]研究可为建立高效、实用的蜚蠊多糖提取途径提供技术参考,同时也为美洲大蠊的保健药品及功能性食品开发提供可靠的科学依据。[Objective]To optimize extraction technique of polysaccharide from Periplaneta americana by papain. [Method]Using different gradient papain to conduct extraction test of Periplaneta americana polysaccharide,using single factor and orthogonal test method,effects of optimal enzyme dosage,extraction time,temperature on polysaccharide content were investigated,the extraction technique of Periplaneta americana polysaccharide was obtained. [Result] The optimal conditions were enzyme dosage 300 μg / g,extraction temperature 60 ℃,extraction time 1. 0 h.[Conclusion]The study can provide technical reference for establishing high efficient,practical Periplaneta americana polysaccharide extraction way,as well as provide reliable scientific basis for development of health medicine and functional food.

关 键 词：蜚蠊多糖 酶解法 提取工艺 条件优化 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]