泄浊解毒方对慢性非特异性溃疡性结肠炎大鼠结肠黏膜环氧合酶-2及前列腺素E_2的影响  

Study of Xiezhuo- jiedu formula on Cycloxygenase-2 and prostaglandin E_2 of intestinal mucosa in chronic nonspecific ulcerative colitis rats

作　　者：刘建平[1] 李玲玲 郎晓猛[1] 任杰[1] 赵源[3] 杨倩[4] 

机构地区：[1]河北省中医院脾胃病一科,河北石家庄050011 [2]河北省直属机关第二门诊部中西医结合科,河北石家庄050051 [3]河北医科大学研究生学院,河北石家庄050071 [4]河北省中医院脾胃病二科,河北石家庄050011

出　　处：《河北中医》2015年第12期1832-1835,共4页Hebei Journal of Traditional Chinese Medicine

基　　金：2014年度河北省自然科学基金资助项目(编号:H2014206462)

摘　　要：目的观察泄浊解毒方对慢性非特异性溃疡性结肠炎(CUC)大鼠结肠黏膜中环氧合酶-2(COX-2)、前列腺素E2(PGE2)表达的影响,探究其可能的作用机制。方法将40只雄性Wistar大鼠适应性饲养1周后,随机分为5组,即空白组、模型组、柳氮磺胺吡啶组、泄浊解毒方高剂量组、泄浊解毒方低剂量组,每组各8只。除空白组外,其余4组大鼠以三硝基苯磺酸(TNBS)联合乙醇造模法复制CUC动物模型。造模成功后第5 d,空白组及模型组大鼠予0.9%氯化钠注射液5 m L灌胃,柳氮磺胺吡啶组予柳氮磺胺吡啶混悬液0.3 g/kg灌胃,泄浊解毒方高、低剂量组分别予20.0、10.0 g/kg泄浊解毒方药液灌胃,均每日1次,连续给药14 d。用药结束后以免疫组化法测定大鼠结肠黏膜COX-2及PGE2的表达。结果模型组大鼠结肠黏膜COX-2及PGE2表达水平均较空白组明显提高(P<0.05);与模型组比较,泄浊解毒方高剂量组、低剂量组及柳氮磺胺吡啶组大鼠结肠黏膜COX-2及PGE2表达水平明显下降(P<0.05),且泄浊解毒方高剂量组低于柳氮磺胺吡啶组(P<0.05)。结论泄浊解毒方治疗CUC大鼠效果良好,抑制COX-2表达、减少PGE2生成可能是其作用机制。Objective To observe the effect and possible mechanism of Xiezhuo -jiedu formula on the expression of cycloxygenase - 2 （ COX - 2） and prostaglandin E2 （ PGE2 ） in colon tissues of chronic nonspecific ulcerative colitis rats. Methods After 40 experimental rats were allowed to acclimate for 1 week,these rats were randomly divided into blank group, model group, salazosulfapyridine group, Xiezhuo- jiedu high- dose group and Xiezhuo- jiedu low - dose group, 8 rats in each group. Expect blank group, rats in other four groups were constructed in chron- ic nonspecific ulcerative colitis model through trinitrobenzene sulfonic acid （TNBS） combined ethanol mode method. Five days after modeling, rats in blank and model group were received 0. 9% sodium chloride injection （5 mL） by gavage, rats in salazosulfapyridine group were received salazosulfapyridine suspension （0.3 g/kg） by gavage. Rats in Xiezhuo- jiedu high- dose and low- dose group were received Xiezhuo -jiedu formula at a dose of 20.0 g/kg and 10.0 g/kg respectively. All rats were administered once a day continuously for 14 d. The expression of COX - 2 and PGE2 in Colon tissues was detected by immunohistochemical method after administration. Results The expression ofCOX- 2 and PGE2 of colon tissnes in model group was obviously higher than that in blank group （P 〈 0.05 ）. As compared with model group, the expression of COX - 2 and PGE2 in Xiezhuo- jiedu high- dose, Xiezhuo- jiedu low- dose and salazosulfapyridine group were obviously decreased （P 〈 0.05 ）, and the decrease in Xiezhuo -jiedu high - dose group was obviously lower than that in salazosulfapyridine group （P 〈 0. 05）. Conclusion Xiezhuo - jiedu formula has satisfactory treatment effect in chronic nonspecific ulcerative cotitis rats. Its mechanism may be involved to suppress the expression of COX - 2 and reduce PGE2 generation.

关 键 词：结肠炎 溃疡性 大鼠 解毒剂(中药) 动物实验 前列腺素E类 

分 类 号：R574.621[医药卫生—消化系统] R285.5[医药卫生—内科学]