男乳癌与AR基因CAG重复多态性的相关性研究  

Related research of male breast cancer and CAG repeat polymorphism of AR gene

作　　者：崔佳琳[1] 黄睿[2] 姜永冬[3] 韩继广[3] 牛明[3] 魏巍[3] 郑伟[3] 宋燕妮[3] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院检验科,哈尔滨150081 [2]哈尔滨医科大学附属第二医院普外科 [3]哈尔滨医科大学附属肿瘤医院乳腺外科

出　　处：《实用肿瘤学杂志》2015年第6期492-496,共5页Practical Oncology Journal

基　　金：黑龙江省卫生厅课题（2013066）;黑龙江省自然基金课题资助（H2015050）;国家自然基金课题资助（H81302286）

摘　　要：目的探讨雄激素受体（AR）基因外显子CAG重复频数多态性与男性乳腺癌（男乳癌）发病风险的关系。方法研究对象为40例男性乳腺癌患者和40例男性健康者，从外周血提取DNA，对AR基因外显子CAG编码序列进行PCR扩增、测序和计算GAG重复频数，用χ2检验和Logistic回归分析AR基因CAG重复频数长度对男乳癌发病风险的影响。结果男性乳腺癌病例组和对照组CAG重复频数长度存在差异，其差异具有统计学意义，CAG重复频数长度超过22男性乳腺癌发病风险是重复颇数长度少于21的3．52倍（OR=3．52，P=0．036）。结论AR基因CAG重复频数长度是预测男性乳腺癌发病风险的指标，较长（超过22）的CAG重复序列可增加男乳癌的发病风险。Objective To investigate the correlation between（CAG） n repeat pelymorphism of androgen receptor（AR） gene and male breast cancer. Methods 40 cases of male breast cancer and 40 controls were col- lected. DNA was extracted from peripheral blood and the AR gene CAG coding exon sequences for PCR amplifica- tion, sequencing and calculated the number of CAG repeats frquency.χ2 test and Logistic regression analysis were used assess the AR gene CAG repeat length frequency affect the number of male breast cancer risk. Results There was statistieaUy significant difference in male breast cancer cases and controls the number of CAG repeat length frequency. Man for whom the（ CAG）n≥22 repeat sequence had 3.52 times risk of male breast compared （CAG） n ≤22 （ OR = 3.52,P = 0.036）. Conclusion AR gene CAG repeat length is a predictor of the frequency of male breast cancer risk. Longer CAG repeats can increase the risk of male breast cancer.

关 键 词：男性乳腺癌 AR CAG重复频数 多态性 

分 类 号：R73[医药卫生—肿瘤]