FCGR3A基因多态性对西妥昔单抗介导抗体依赖的细胞介导的细胞毒作用杀伤A549细胞的影响  

Effect of FCGR3A Polymorphisms on Antibody-dependent Cetuximab-mediated Cytotoxicity in A549 Cells

作　　者：李瑾昱[1] 朱艳云[1] 张国庆[1] 孙胜杰[1] 焦顺昌[1] 

机构地区：[1]中国人民解放军总医院肿瘤内一科,北京100853

出　　处：《中国医学科学院学报》2015年第6期645-649,共5页Acta Academiae Medicinae Sinicae

基　　金：军队十一五重点课题(06G106)~~

摘　　要：目的探讨FCGR3A基因多态性对西妥昔单抗介导抗体依赖的细胞介导的细胞毒(ADCC)作用杀伤A549细胞的影响。方法选取肺腺癌A549细胞作为靶细胞,NKTm细胞为效应细胞。基因测序法检测NKTm细胞FCGR3A基因多态性,CCK-8法检测西妥昔单抗介导NKTm细胞的ADCC活性。结果 NKTm细胞FCGR3A具有基因多态性,分别为V/V、V/F及F/F表型。西妥昔单抗介导3种表型的NKTm细胞的ADCC活性差异具有统计学意义(P=0.0015),其中FCGR3A-158V/V表型的NKTm细胞的ADCC活性比V/F及F/F表型强(P<0.01),而V/F与F/F表型的NKTm细胞ADCC活性差异无统计学意义(P>0.05)。结论西妥昔单抗介导的NKTm细胞ADCC活性与FCGR3A基因多态性有关。Objective To investigate the effect of FCGR3 A polymorphisms on the antibody-dependent cell-mediated cytotoxicity（ ADCC） activity induced by cetuximab against A549 cells. Methods A549 cell line was used as target cells and NKTm cells as effector cells. FCGR3 A polymorphisms were detected by direct sequencing. The ADCC activity mediated by cetuximab was assessed by CCK-8 assay. Results Three genotypes of FCGR3 A were detected： V / V,V / F,and F / F. The ADCC activity of NKTm cells with these three different genotypes mediated by cetuximab were significantly different（ P = 0. 0015）. NKTm cells with FCGR3A-158 V / V genotypes had significantly higher ADCC activity than FCGR3A-V / F or F / F genotypes（ P〈0. 01）,whereas the ADCC activity between V / F and F / F genotype showed no statistical significance（ P〉0. 05）. Conclusion FCGR3 A polymorphisms have an impact on ADCC activity mediated by cetuximab in NKTm cells.

关 键 词：西妥昔单抗 A549细胞 FCGR3A 抗体依赖的细胞介导的细胞毒作用 

分 类 号：R735.5[医药卫生—肿瘤]