IGF2BP3对油酸诱导的L-O2肝细胞脂肪变性的影响  

The influence of IGF2BP3 on lipid metabolism in L-O2 fatty liver cell

作　　者：宋玉萍[1] 刘志民[2] 范晓芳[1] 张宁[1] 李雪松[1] 郑徽[1] 范玉娟[1] 沈甲乙[1] 张钰[1] 沈春芳[1] 杨架林[1] 

机构地区：[1]复旦大学附属闵行医院/闵行区中心医院内分泌科,上海201199 [2]第二军医大学附属长征医院内分泌科

出　　处：《中华内分泌代谢杂志》2015年第12期1060-1063,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：基金项目：国家重点基础研究发展计划（“973”计划,2005CB523304）

摘　　要：探讨胰岛素样生长因子2信使RNA结合蛋白3（IGF2BP3）在非酒精性脂肪性肝病形成中的作用及可能机制。本研究利用油酸刺激L—O2诱导脂肪肝模型，并通过干扰IGF2BP3基因表达后，观察相关生化指标和油红O染色观察脂滴形成的变化。与对照组细胞相比，脂肪肝细胞模型组IGF2BP3mRNA表达水平明显升高（P〈0．05），生化指标丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、三酰甘油、肿瘤坏死因子α及白细胞介素6等水平明显上升（均P〈0．05）。经干扰IGF2BP3基因表达后，ALT、AST、三酰甘油、肿瘤坏死因子仅、白细胞介素6等水平明显下降（均P〈0．05），经油红染色后，观察到脂肪肝细胞脂滴形成得到明显改善。IGF2BP3可能参与了非酒精性脂肪性肝病的形成与发展。To explore the role and mechanism of insulin-like growth factor 2 mRNA-binding protein 3 （IGF2BP3） formed in non-alcoholic fatty liver disease. By using oleic acid stimulation L-O2 to induce fatty liver model, we observe the changes of relevant biochemical indexes by interference of IGF2BP3 gene expression, to investigate the change of the formation of lipid droplets through oil red O staining. Compared with control cells, in fatty liver cell model group, IGF2BP3 expression level increased significantly （P 〈 0. 05 ）. As to the biochemical indicators： alanine transaminase （ALT）, aspertate aminotransferase （AST）, triglycerides, and tumor necrosis factor-a, interleukin 6 levels were significantly increased （ all P〈O. 05 ）. But, after interference of IGF2BP3 gene expression, ALT, AST, triglycerides, tumor necrosis factor-or, and interleukin 6 levels were significantly decreased （ all P〈O. 05 ）. Fatty droplet formation infatty liver cells were dramatically improved. IGFEBP3 may participate in the formation and development of non-alcoholic fatty liver disease.

关 键 词：IGF2BP3 非酒精性脂肪性肝病 干扰 

分 类 号：R575[医药卫生—消化系统]