血管生成拟态在子宫内膜癌组织中的表达及意义  

Expression and significance of vasculogenic mimicry in endometrial carcinoma tissues

作　　者：梁军[1] 苗蕊[1] 邢慧敏[2] 

机构地区：[1]解放军白求恩国际和平医院妇科,石家庄市050082 [2]石家庄市妇幼保健院妇产科

出　　处：《中国肿瘤临床》2015年第23期1124-1127,共4页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨血管生成拟态(vasculogenic mimicry,VM)在子宫内膜癌中的表达及其与临床病理特征和预后的关系。方法:收集解放军白求恩国际和平医院2005年1月至2014年6月术后随访资料完整的子宫内膜癌标本267例,采用CD31/PAS双重染色鉴定VM结构,分为VM阳性组与VM阴性组,免疫组织化学SP法检测CD133的表达。结果:267例子宫内膜癌患者中65例(24.3%)存在VM。VM的形成与子宫内膜癌FIGO分期(χ2=9.987,P=0.002),组织学分级(χ2=11.795,P=0.001),肌层浸润深度(χ2=5.499,P=0.019),脉管内有无癌栓(χ2=22.599,P<0.001)及淋巴结有无转移(χ2=7.848,P=0.005)密切相关。Kaplan-Meier法生存分析发现VM阳性组生存时间(中位生存时间为51个月)明显低于VM阴性组(中位生存时间为100个月),二者比较差异具有统计学意义(χ2=70.973,P<0.001)。CD133在VM阳性组的表达率为75.4%(49/65),较VM阴性组58.9%(119/202)高,二者比较差异具有统计学意义(χ2=5.720,P=0.017)。结论:VM与子宫内膜癌的发生、发展及恶性度密切相关,是影响患者预后的重要指标,CD133表达阳性的肿瘤干细胞可能参与子宫内膜癌VM的形成。Objective： To investigate the expression of vasculogenic mimicry （VM） in endometrial carcinoma tissues and its rela- tionship with the clinicopathologic features and prognosis of the disease. Methods： A total of 267 paraffin-embedded endometrial carci- noma specimens of patients with complete follow-up data were collected from the Shijiazhuang Bethune International Peace Hospital between January 2005 and June 2014. CD31-PAS dual staining was performed to identify the VM structure. Tissue samples were then divided into VM-positive and VM-negative groups. CD133 expression was detected by immunohistochemical streptavidin peroxidase method. Results： Among the 267 endometrial carcinoma patients, 65 cases （24.3%） were VM positive. VM formation was closely corre- lated with the International Federation of Gynecology and Obstetrics Staging （x2=9.987, P=-0.002）, histodifferentiation grade （x^2= 11.795, P=-0.001）, myometrial invasion depth （x2=5.499, P=0.019）, vascular cancer embolus （x2=22.599, P〈0.001）, and lymph node me- tastasis （x^2=7.848, P=-0.005）. Kaplan-Meier survival curve analysis showed that survival time was significantly shorter in the VM-posi- tive group （median survival time was 51 months） than in the VM-negative group （median survival time was 100 months） （x2=70.973, P〈 0.001）. Moreover, CD133 expression was significantly higher in the VM-positive group [75.4% （49/65）] than in the VM-negative group [58.9% （119/202）] （x2=5.720, P=0.017）. Conclusion： VM is closely correlated with the pathogenesis, development, and malignancy of endometrial carcinoma. Furthermore, VM is one of the important indexes influencing the prognosis of this disease. Therefore, CD133- positive ceils may contribute to VM formation.

关 键 词：子宫内膜癌 血管生成拟态 免疫组织化学 预后 

分 类 号：R737.33[医药卫生—肿瘤]