不同激素配比对半支莲细胞生长与甜菜红素积累及抗氧化酶活性的影响  

Cell Growth,Betacyanin Accumulation and Antioxidant Enzyme Activities of Portulaca grandiflora Hook Under Different Plant Regulators

作　　者：赵胡[1] 王玉娇[1] 管恒洁 刘雪[1] 

机构地区：[1]阜阳师范学院生物与食品工程学院,阜阳236037

出　　处：《天然产物研究与开发》2015年第12期2014-2019,共6页Natural Product Research and Development

基　　金：安徽省教育厅自然科学基金(2015KJ006)

摘　　要：研究了不同激素配比对培养30 d的半支莲细胞生长量、甜菜红素含量及细胞内抗氧化酶活性的影响。结果表明:半支莲细胞培养的最佳激素配方为1.0 mg/L 2,4-D+0.5 mg/L 6-BA,有利于半支莲细胞干重增加和甜菜红素积累。向培养基中添加2,4-D可显著提高半支莲细胞参与活性氧代谢的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,这一结果暗示活性氧(ROS)有可能参与了2,4-D诱发的半支莲细胞中甜菜红素合成的信号转导途径。本研究为进一步利用细胞培养技术生产甜菜红素以及研究甜菜红素在细胞内的生物合成调节机制奠定了基础。This experiment was designed to investigate cell growth, betacyanin accumulation and antioxidant enzyme activities changes of Portulaca grandiflora under different plant regulators. The results were shown as follows ： Best cell growth and betacyanln accumulation was observed in MS medium containing 1.0 mg/L 2,4-D and 0.5 mg/L 6-BA. 2,4- D significantly enhanced the activities of superoxide dlsmutase （SOD） ,peroxidase （POD） and catalase （CAT） involved in reactive oxygen species （ROS） metabolism, suggesting that ROS mediated signal transduction pathway prominently exists in 2,4-D-induced betacyanin biosynthesis in cells of P. grandiflora. These results laid the foundation for further producing betaeyanin by plant cell culture and studying betacyanin biosynthesis regulation mechanism in plant cell.

关 键 词：半支莲 细胞培养 2 4-D 甜菜红素 抗氧化酶 

分 类 号：S792.12[农业科学—林木遗传育种]