miR-136通过抑制PPP2R2A表达调控胃癌细胞SGC7901凋亡  

miR-136 regulated apoptosis in gastric cell SGC7901 by suppressing PPP2R2A

作　　者：邢亚楠[1] 邓鹏[1] 徐惠绵[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤外科,沈阳110001

出　　处：《临床与病理杂志》2015年第12期2108-2111,共4页Journal of Clinical and Pathological Research

基　　金：辽宁省科技计划项目(2011225019)~~

摘　　要：目的:检测miR-136对胃癌细胞SGC7901增殖和凋亡的影响及其机制。方法:分别利用MTT实验和Annex in V-PI实验检测转染miR-136 inhibitor的胃癌细胞SGC7901增殖和凋亡。利用western blot和双荧光素酶报告基因技术检测miR-136的靶基因。结果:抑制miR-136表达可以明显抑制SGC7901细胞增殖和诱导其凋亡。miR-136能够与PPP2R2A mRNA 3'-UTR区结合。结论:抑制miR-136表达能够促进PPP2R2A蛋白表达,进而引起胃癌细胞凋亡。Objective： To detect the effects and mechanisms ofmiR-136 on gastric cancer cell SGC7901. Methods： The proliferation and the apoptosis of SGC7901 cells after transfected with miR-136 inhibitor were detected by M＇IT assay and Annexin V-PI assa）5 respectively. Western blot and dual luciferase reporter gene were used to detect the target gene of miR-136. Results： Inhibition of miR-136 could inhibit the proliferation and induce apoptosis in SGC7901 cells, miR-136 could combine with 3＇-UTR of PPP2R2A mRNA. Conclusion： Inhibition of n＇fiR-136 could induce PPP2R2A expression and apoptosis in SGC7901 cells.

关 键 词：SGC7901细胞 miR-136 PPP2R2A 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]