胃肠道间质瘤中环氧合酶2基因的表达  

Expression of cyclooxygenase 2 in gastrointestinal stromal tumors

作　　者：李小红[1] 成元华[1] 郭立新[2] 

机构地区：[1]贵州医科大学病理学教研室,贵州省贵阳市550004 [2]四川大学华西医院病理科,四川省成都市610041

出　　处：《世界华人消化杂志》2015年第35期5628-5635,共8页World Chinese Journal of Digestology

基　　金：贵州省科学技术基金资助项目;Nos.黔科合J字[2009]2187号;黔科合J字LKG[2013]27号;贵州省科技计划基金资助项目;No.黔科合SY字[2011]3062号~~

摘　　要：目的:探讨胃肠道间质瘤(gastrointestinal stromal tumors,GISTs)中环氧合酶2(cyclooxygenase 2,COX2)基因的蛋白及mRNA表达与临床病理特征及预后之间的关系.方法:采用免疫组织化学EnVision两步法检测GISTs病例肿瘤组织中COX2蛋白的表达情况;采用原位杂交方法检测GISTs中COX2mRNA的表达情况;并对患者进行随访.结果:74例GISTs中,COX2蛋白阳性表达62例,阳性表达率为83.8%;COX2 mRNA阳性表达58例,阳性表达率为78.4%.COX2蛋白及mRNA表达情况在GISTs不同危险度分组间比较差异有统计学意义(P<0.05),在高危险度组中阳性表达率最高,而在不同性别、年龄、发生部位、组织学类型及是否转移组间比较差异均无统计学意义(P>0.05).38例患者获访,随访率51.4%;COX2蛋白强阳性表达(+++)24例,其中5例转移,2例死亡;其余阴性(-)、弱阳性(+)及中度阳性(++)表达病例均未发现复发、转移或死亡.COX2mRNA强阳性表达(+++)17例,其中3例转移,2例死亡;阴性表达(-)6例,未发现复发、转移或死亡;而弱阳性(+)及中度阳性(++)表达病例中均有1例发生转移,未发现复发及死亡.结论:GISTs中COX2蛋白及mRNA的表达上调提示COX2可作为评估GISTs危险度分级的另一潜在指标,并且可能影响患者预后.AIM： To investigate the correlation of cyclooxygenase 2 （COX2） protein and mRNA expression with clinicopathological characteristics and prognosis in gastrointestinal stromal tumors （GISTs）. METHODS： EnVision technique was used to detect COX2 protein expression in GISTs and in situ hybridization method was used to detect COX2 mRNA expression. The patients were followed for prognosis evaluation. RESULTS： Of 74 cases of GISTs, positive expression of COX2 protein was observed in 62 （83.8%） cases and positive expression of COX2 mRNA was observed in 58 （78.4%）. COX2 protein and mRNA expression between different risk groups was statistically significant （P 〈 0.05）, with the highest positive expression in the high risk group. COX2 protein and mRNA expression had no significant correlation with gender, age, lesion location, histological type, or metastasis （P 〉 0.05）. A total of 38 （51.4%） patients were followed; of 24 patients with strongly positive expression of COX2 protein, 5 had metastases and 2 died. No recurrence, metastasis or death was observed in cases with negative, weakly positive or moderately positive expression. Of 17 cases showing strongly positive expression of COX2 mRNA, 3 had metastases and 2 died. No recurrence, metastasis or death was found in all 6 cases with negative expression of COX2 mRNA. Metastasis was observed in a case with weekly positive expression and a case with moderately positive expression, with no recurrence or death. CONCLUSION： Upregulation of COX2 protein and mRNA expression in GISTs suggests that COX2 is a possible indicator for GISTs risk stratification and might affect prognosis.

关 键 词：胃肠道间质瘤 环氧合酶2 免疫组织化学 原位杂交 预后 

分 类 号：R735[医药卫生—肿瘤]