姜黄素对胃癌细胞抑癌基因甲基化的抑制作用  

Inhibitory Effects of Curcumin on the Methylation of Tumor Suppressor Genes in Gastric Cancer Cells

作　　者：陈馨[1] 肖乐[1] 吴小雪[1] 姚群峰[1] 

机构地区：[1]湖北中医药大学基础医学院,武汉430065

出　　处：《华中科技大学学报（医学版）》2015年第6期674-677,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省自然科学基金资助项目(No.2013CFB068)

摘　　要：目的探讨姜黄素对肿瘤细胞p16和MGMT基因甲基化的抑制作用。方法采用不同浓度的姜黄素（20、40、60μmol/L）分别处理人胃癌SGC-7901细胞,MTT法检测细胞的增殖;利用甲基化特异性PCR（Methylation-specific PCR,MSP）法检测不同浓度姜黄素溶液作用前后肿瘤细胞p16和MGMT基因甲基化状态的改变及其对mRNA表达的影响。结果姜黄素能够抑制SGC-7901细胞增殖和生长,肿瘤细胞中的p16和MGMT基因启动子区均呈过甲基化状态,低浓度（20μmol/L）的姜黄素对肿瘤细胞无明显的去甲基化作用,高浓度（40~60μmol/L）的姜黄素对体外培养的肿瘤细胞呈去甲基化效应,并使p16和MGMT基因的mRNA表达增强。结论姜黄素对SGC-7901细胞的增殖具有明显抑制作用,高浓度的姜黄素溶液对p16和MGMT基因具有甲基化抑制作用,并促进基因表达。Objective To explore the inhibitory effects of curcumin on the methylation of tumor suppressor genes p16 and MGMT. Methods Gastric cancer ceils SGC-7901 were treated with different concentrations of curcumin（20, 40, 60 μmol/L）. The proliferation of tumor cells was detected by MTT assay. The methylation status of the p16 and MGMT genes in tumor cells before and after treatment with different concentrations of eurcumin was detected by methylation-speeific PCR（MSP）. Results Curcumin could inhibit the proliferation of SGC 7901 cells. The promoters of p16 and MGMT genes were hypermethylated in SGC-7901 cells. Low concentration（20 μmol/L）.of curcumin had no significant demethylation effects on tumor cells, and high concentration（40--60 μmol/L） of curcumin caused demethytation of tumor ceils and increased the mRN A expression levels of p16 and MGMT genes. Conclusion Curcumin significantly inhibits the proliferation of SGC-7901 cells. Cureumin at high con- centration suppresses the methylation of p16 and MGMT genes and promotes the gene expressions in gastric cancer ceils.

关 键 词：姜黄素 P16基因 MGMT基因 DNA甲基化 

分 类 号：R735.2[医药卫生—肿瘤]