地黄提取物对高尿酸血症小鼠的影响  

Effect of Radix Rehmanniae extract on hyperuricemia mice

作　　者：刘静 刘梅 杨波 马静 鲁毅 邱彦 

机构地区：[1]解放军454医院药剂科,江苏南京210002

出　　处：《中国药物应用与监测》2015年第6期347-350,共4页Chinese Journal of Drug Application and Monitoring

基　　金：南京军区医学科研基金资助项目(09Z014)

摘　　要：目的:观察地黄提取物对高尿酸血症小鼠血清尿酸水平的影响,初步探讨其可能的作用机理。方法:采用氧嗪酸钾腹腔注射诱导高尿酸血症小鼠模型,将成模小鼠随机分为模型组、地黄提取物低(2 g·kg^(-1))、中(4 g·kg^(-1))、高(8 g·kg^(-1))剂量组和别嘌醇(20 mg·kg^(-1))组。对小鼠血清尿酸、肌酐、一氧化氮,肝脏黄嘌呤氧化酶(XOD)和腺苷脱氨酶(ADA)等指标进行观察分析,并对小鼠肾脏进行病理检查。结果:给药干预14 d后,与模型组比较,地黄提取物中、高剂量组均可显著降低高尿酸血症小鼠血清尿酸、肌酐含量(P<0.01),显著升高一氧化氮水平(P<0.05,P<0.01),并可显著抑制高尿酸血症小鼠肝脏XOD活性(P<0.01)。地黄提取物高剂量组对高尿酸血症小鼠肝脏ADA活性有显著的抑制作用(P<0.05)。病理检查结果显示,地黄提取物三个剂量组的肾脏组织病变程度要明显轻于模型组。结论:地黄提取物对高尿酸血症小鼠具有降低血清尿酸水平作用,其机制与抑制肝脏XOD和ADA活性有关。Objective： To observe the effect of Radix Rehmanniae extract on the level of serum uric acid in hyperuricemia mice and investigate the mechanism preliminarily. Methods： The intraperitoneal injection of uricase inhibition potassium oxonate was used to induce the hyperuricemia in mice. The mice were randomly divided into model group, Radix Rehmanniae extracts treatment groups（with the doses of 2, 4 and 8 g·kg-1） and the allopurinol positive control group（20 mg·kg-1）. The serum levels of uric acid, creatinine, nitric oxide, and the levels of xanthine oxidase（XOD） and adenosine deaminase（ADA） in liver were observed, and the pathological changes in kidney of mice were detected. Results： After 14 days of drug intervention, compared with the model group, the Radix Rehmanniae extract middle and high dose groups reduced the levels of serum uric acid and creatinine（P 〈 0.01）, increased serum levels of nitric oxide（P 〈 0.05, P 〈 0.01）, and signifi cantly inhibited the liver XOD（P 〈 0.01）. It showed that Radix Rehmanniae extract high dose group signifi cantly inhibited the ADA in hyperuricemia mice（P 〈 0.05）. The results of pathological examination showed that the pathological changes in kidney of all extracts groups were lower than that of the model group. Conclusion： The Radix Rehmanniae extract can reduce the level of serum uric acid in the model of hyperuricemia mice, and the effect is related to the inhibition of the activity of liver XOD and ADA.

关 键 词：地黄提取物 高尿酸血症 尿酸 黄嘌呤氧化酶 腺苷脱氨酶 小鼠 

分 类 号：R285.5[医药卫生—中药学]