电场对脂肪酶二级结构及其活性的影响  

Study on the Effect of Electric Field on the Secondary Structure and Activity of Lipase

作　　者：敖敦格日乐[1] 杨体强[2] 包斯琴高娃[3] 石磊[1] 

机构地区：[1]内蒙古医科大学计算机信息学院,内蒙古呼和浩特010110 [2]内蒙古大学物理科学与技术学院,内蒙古呼和浩特010021 [3]内蒙古工业大学理学院,内蒙古呼和浩特010051

出　　处：《食品与生物技术学报》2015年第12期1256-1261,共6页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(30260028);内蒙古自治区重大科技项目(200508010101)

摘　　要：脂肪酶被不同强度的电场处理5 min,用红外光谱法研究电场对其二级结构的影响,并测定酶活性的变化。实验结果显示：在1.0~6.0 kV/cm范围,电场作用对脂肪酶二级结构单元相对含量的影响程度不同。与对照组相比,处理组的α-螺旋和β-折叠含量普遍减少,减少幅度分别为4.9%~10.9%和3.2%~22.6%;β-转角和无规卷曲含量普遍增加,增加幅度分别为12.5%~37.7%和3.1%~24%。并且各种结构的含量在3.0 kV/cm电场强度条件下变化最为明显。经电场处理后脂肪酶活性显著增加,这与β-转角和无规卷曲含量的增加有一定的相关性。The changes in the secondary structures and activity of lipase after 5 min treatment using electric field with different strength were studied by FTIR（Fourier-transformation infrared spectroscopy）. The effect of electric field on the relative contents of the lipase secondary structures,i.e. α-helix,β-sheet,β-turn and random coil,varied within the range of 1.0 kV/cm to 6.0 kV/cm.Compared with the control groups,the relative contents of α-helix and β-sheet both decreased,namely,4.9%~10.9% for α-helix and 3.2%~22.6% for β-sheet respectively. The relative contents of β-turn and random coil increased instead,namely,12.5%~37.7% for β-turn and 3.1%~24% for random coil respectively. The most significant change of the relative contents for different secondary structure was observed at the 3.0 kV/cm electric field,which suggested the lipase activity was greatly improved after treatment by electric field. The improvement of the activity was related to the content increase of β-turn and random coil.

关 键 词：电场 脂肪酶 红外光谱 二级结构 酶活性 

分 类 号：Q68[生物学—生物物理学]