长链非编码RNA CCAT2在肾癌中的表达和肾癌临床分期的关系  

Expression level of lnc RNA CCAT2 is related with the clinical stage of RCC

作　　者：胡祎民[1,2] 金露[1,2,3] 李一帆[1,2,3] 陈独群 苏郑明[1,2] 刘家驹[1,2] 杨尚琪[1,2] 桂耀庭[2] 毛向明[1,2] 来永庆[1,2] 

机构地区：[1]北京大学深圳医院泌尿外科,广东省518036 [2]北京大学深圳医院男性生殖实验室,广东省518036 [3]安徽医科大学,合肥230032

出　　处：《中华临床医师杂志（电子版）》2015年第21期38-41,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金(81101922);广东省医学科研基金(A2012584;A2013606);2013年深圳市科技研发资金知识创新计划基础研究项目(JCYJ20130402114702124);广东省重点医学专科经费

摘　　要：目的检测并分析长链非编码RNA CCAT2在肾癌中的表达水平及其表达与患者临床特征之间的关系。方法通过实时荧光定量PCR检测肾癌及癌旁正常组织、肾癌细胞系(ACHN、786-O)及人胚肾细胞系(293T)中长链非编码RNA CCAT2的表达水平,通过配对t检验比较配对组织间表达差异;列出四格表,通过Fisher确切概率法探究CCAT2表达水平与患者临床特征之间的关系。结果 32例肾癌组织中26例(81.25%)表达升高,CCAT2在肾癌组织中表达水平明显升高(P<0.01),正常组织中CCAT2相对表达量为1±0.205 85,肾癌组织中CCAT2相对表达量为12.817 12±0.551 67;CCAT2在293T细胞中表达水平明显低于786-O、ACHN细胞(P<0.01),293T细胞中CCAT2相对表达量为1±0.004 88,786-O细胞中CCAT2相对表达量为15.670 72±1.075 72,ACHN细胞中CCAT2相对表达量为36.504 44±8.990 78;CCAT2在临床分期、分级较高的肾癌组织中升高更明显(P<0.05)。未发现CCAT2表达水平和患者年龄、性别、肾癌组织类型之间的相关性(P>0.05)。结论肾癌组织中CCAT2表达升高,提示CCAT2与肾癌发生相关。Objective To detect and analyze the expression level of lncRNA CCAT2 in RCC and explore the correlations between the level of CCAT2 and the clinicopathological characteristics. MethodsQRT-PCR was performed to detect the expression of CCAT2 in RCC tissues, paired normal tissues, RCC cell lines (ACHN, 796P) and human embryo kidney cell line (293T) andt test was performed to find the differences of CCAT2 in paired RCC tissues. The relationships between the level of CCAT2 and the clinicopathological characteristics were explored by Fisher’s exact probability.ResultsCCAT2 was overexpressed in 26 (81.25%) RCC tissues and was significantly up-regulated in RCC tissues. The relative expression level of CCAT2 in normal tissues was 1±0.205 85, and in RCC tissues the expression level was 12.817 12±0.551 67. The expression of CCAT2 in 293T cell line was significantly lower (P<0.01) than 786-O and ACHN cell line.The relative expression level was 1±0.004 88 in 293T cell line, 15.670 72±1.075 72 in 786-O cell line and 36.504 44±8.990 78 in ACHN cell line, respectively. The expression level of lncRNA CCAT2 was concerned with the clinical stage of RCC (P<0.05). No relationship was found between the expression of CCAT2 and the age, sex of patients or the subtype of RCC (P>0.05). ConclusionCCAT2 was up-regulated in RCC tissues, indicating that CCAT2 was related&nbsp;with the development of RCC.

关 键 词：肾肿瘤 CCAT2 

分 类 号：R737.11[医药卫生—肿瘤]