CXCL12对人胃癌细胞增殖及受体CXCR4和CXCR7表达的影响  

Effects of CXCL12 on the proliferation of gastric cancer cells and expression of chemokine receptors CXCR4 and CXCR7

作　　者：朱妍[1] 王溪唯 朴花子[1] 

机构地区：[1]延边大学医学院药理学教研室,吉林延吉133002

出　　处：《延边大学医学学报》2015年第3期168-171,共4页Journal of Medical Science Yanbian University

基　　金：吉林省自然科学基金(20125236)

摘　　要：[目的]探讨趋化因子CXCL12对人胃癌SGC-7901细胞增殖及趋化因子受体CXCR4,CXCR7和血管内皮生长因子(VEGF)、基质金属蛋白(MMP)-2,MMP-9mRNA表达的影响.[方法]取体外培养的人胃癌SGC-7901细胞,用不同质量浓度的CXCL12(0.1,1.0,10.0,100.0μg/L)处理后培养72 h,采用MTT法检测人胃癌SGC-7901细胞的增殖率;用CXCL12(10.0μg/L)处理人胃癌SGC-7901细胞48h后,应用RT-PCR检测细胞内CXCR4,CXCR7,VEGF,MMP-2及MMP-9 mRNA的表达水平.[结果]不同质量浓度的CXCL12均可明显促进人胃癌SGC-7901细胞的增殖率(P<0.001),CXCL12可显著上调CXCR4及CXCR7 mRNA的表达(P<0.001,P<0.01),增加VEGF,MMP-2及MMP-9 mRNA的表达(P<0.05).[结论]趋化因子CXCL12可促进人胃癌SGC-7901细胞的增殖,其机制可能与上调CXCR4及CXCR7表达,从而增加VEGF,MMP-2及MMP-9表达有关.OBJECTIVE To study the effects of CXCL12 on the proliferation of gastric cancer cell line SGC-7901 and the mRNA expression of CXCR4,CXCR7,VEGF,MMP-2and MMP-9.METHODS The proliferation rates of SGC-7901 cells cultured with different mass concentration（0.1,1.0,10.0,100.0μg/L）of CXCL12 in vitro for 72 h were detected by MTT,and the mRNA expressions of CXCR4,CXCR7,VEGF,MMP-2 and MMP-9 of SGC-7901 cells cultured with 10.0μg/L of CXCL12 for 48 h were measured by RT-PCR.RESULTS The different mass concentration of CXCL12 increased significantly the proliferation rate of SGC-7901cells（P〈0.001）and upregulated the mRNA expression of CXCR4,CXCR7,VEGF,MMP-2and MMP-9（P〈0.001,P〈0.01,P〈0.05）,respectively.CONCLUSION CXCL12 could promote the proliferation of gastric cancer cells SGC-7901,and its mechanism maybe related to upregulate the CXCR4 and CXCR7to promote the expression of VEGF,MMP-2and MMP-9.

关 键 词：胃肿瘤 CXCL12 细胞增殖 VEGF MMP-2 MMP-9 

分 类 号：R735.2[医药卫生—肿瘤]