大豆CML家族基因的生物信息学分析  

Bioinformatics Analysis of GmCML Genes in Soybean Genome

作　　者：陈超[1] 端木慧子[1] 朱丹[1] 刘艾林[1] 肖佳雷[1] 朱延明[1] 

机构地区：[1]东北农业大学农业生物功能基因重点实验室,黑龙江哈尔滨150030

出　　处：《大豆科学》2015年第6期957-963,共7页Soybean Science

基　　金：转基因生物新品种培育重大专项(2011ZX08004-002);国家自然科学基金(31171578);黑龙江省高校科技创新团队建设计划(2011TD055);国家基础科学人才培养基金(J1210069)

摘　　要：CMLs蛋白是一类具有Ca2＋结合的EF-hand保守结构域蛋白,广泛参与钙依赖的信号传导途径,在植物生长发育及生物胁迫与非生物胁迫响应中起着重要的作用。通过NCBI和植物基因组注释数据库等筛选并获得了68个大豆GmCML蛋白;分析了所有蛋白的基本特性;通过与拟南芥CMLs基因的进化树分析表明,GmCML家族基因属于8个亚类;对GmCML家族基因进行了染色体定位与重复基因进化关系分析,发现其具有较高的进化速率;序列比对发现GmCML类蛋白含有2~4个保守的EF-hand结构域;利用芯片数据对野生大豆在碱胁迫下的叶和根中的CMLs基因表达模式进行了分析,得到26个GsCML基因在碱胁迫下有显著的差异表达,且在根和叶中的差异表达模式不同,表明这些基因参与植物碱胁迫应答,且具组织表达特异性。CMLs is a kind of calcium-binding proteins with conserved EF-hand motifs,play an important role in calcium-dependent signaling pathway and play a key role in response to plant development and abiotic and bioticstresses. In this study,we identified and characterized 68 soybean CML through plant genome annotation and NCBI database. Phylogenetic analysis suggested that these Gm CML genes could be classified into eight groups,combining with Arabidopsis CMLs. Furthermore,physical locations and gene duplications showed a higher evolution rate of Gm CML. Sequence alignment confirmed that this family proteins contain 2-4 conserved EF-hand motifs. Expression profiles of all CML transcripts from bicarbonate stress treated G.soja showed that there were 26 differently expressed Gm CMLs. The transcript pattern in leaves and in roots was different.These results suggest that those genes may play important roles in plant environmental stress responses and adaptation,with different function in leaf and root.

关 键 词：大豆 CMLs EF-HAND 生物信息学 进化分析 

分 类 号：S565.1[农业科学—作物学]