基于抗氧化活性优化金花茶花超声提取工艺  

Optimization of Ultrasound Extraction Process of Camellia chrysantha Chi based on Antioxidant Activity

作　　者：牛广俊 朱思[1] 王维华[1] 魏艺聪[1] 林娜[1] 陈清英 梁一池[1] 

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]漳平市展宏金花茶专业合作社,福建漳平364400

出　　处：《福建林业科技》2015年第4期13-17,共5页Journal of Fujian Forestry Science and Technology

基　　金：福建省教育厅项目(JA11138);福建省龙岩市资助项目(201367)

摘　　要：以金花茶的花为研究对象,探讨乙醇体积分数、料液比、提取时间3个因素对金花茶花超声提取物抗氧化能力的影响;在此基础上,以提取物的抗氧化能力为响应值,采用Box-Benhnken中心组合设计和响应面分析法,优化金花茶花超声提取工艺。结果表明,金花茶花最佳提取工艺为:乙醇体积分数51%、料液比1∶48 g·m L-1,提取时间53 min。在此条件下,提取物抗氧化能力是阳性对照药DL-α-生育酚(0.218 mg·m L-1)的1.5倍。金花茶花提取液中三大抗氧化物质总酚、总黄酮、皂苷的得率分别为:6.25%、17.18%、13.26%。With the antioxidant activity of Camellia chrysantha extracts as indicators,using Box-Benhnken and Response Surface Methodology optimized the ultrasonic extracting procedure,based on the study of the influence of alcohol volume fraction,the ratio of solid to liquid and the extraction duration on the extracts antioxidant ability. Experimental results indicated that the optimum extracting conditions were as following： 51% alcohol,treating with ultrasonic for 53 min and the ratio of solid to liquid was 1 ∶ 48. Under these conditions,the free radical scavenging rate of Camellia chrysantha extracts is 1. 5 times of positive control medicine DL-alphaTocopherol（ 0. 218 mg·m L- 1）. Furthermore,the yield of total phenols,flavonoids and saponin were 6. 25%,17. 18% and 13. 26%.

关 键 词：金花茶 抗氧化能力 响应曲面法 超声提取工艺 

分 类 号：S685.14[农业科学—观赏园艺]