云南地方猪种mtDNA D-loop区遗传多样性研究  

The Genetic Diversity of Mitochondrial DNA D-loop in Yunnan Native Pigs

作　　者：权金强 苟潇[2] 赵生国[1] 

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070 [2]云南农业大学动物科学技术学院,昆明650201

出　　处：《四川农业大学学报》2015年第4期422-428,共7页Journal of Sichuan Agricultural University

基　　金：国家自然科学基金青年基金项目(31101682)

摘　　要：【目的】为揭示云南7个地方猪种遗传多样性及其分布规律。【方法】以云南7个地方猪品种(群体)为对象,研究了线粒体DNA D环高变区(mtDNA D-loop HVI)的序列多态性。【结果】研究表明,在全长435bp的核苷酸序列中共检测到23个多态位点,由此界定了30个单倍型。云南地方猪种整体的单倍型多样度(Hd)和核苷酸多样度(Pi)分别为0.820和0.004 51。在7个品种(群体)中,保山猪单倍型多样度最高(0.828),高黎贡山猪最低(0.644);滇南小耳猪的核苷酸多样度最高(0.005 21),高黎贡山猪同样最低(0.003 27)。由遗传变异贡献率和遗传独特性贡献所决定的总体遗传贡献率中,迪庆藏猪总体遗传贡献率最高(10.342 9),高黎贡山猪和大河猪最低(均为1.059 4)。【结论】就总体单倍型丰富度效应而言,迪庆藏猪、滇南小耳猪、保山猪和撒坝猪对维持云南地方猪种遗传多样性呈正效应,而明光猪、高黎贡山猪和大河猪呈负效应。【Objective】To reveal the genetic diversity and distribution in seven native pig breeds/populations in Yunnan province.【Method】The mitochondrial DNA D-loop hypervariable region（mtDNA D-loop HVI）of Yunnan native pigs were investigated in this study.【Results】The results showed that 23 polymorphism sites were found in the full-length 435 bp which defined 30 haplotypes.The haplotype diversity（Hd）and nucleotide diversity（Pi）of Yunnan seven native pigs were 0.820 and 0.004 51 respectively.Among the seven native pig breeds/populations,the highest value of Hd was found in Baoshan pig（0.828）and the lowest in Gaoligongshan pig（0.644）,the highest value of Pi（0.005 21）was found in Diannanxiaoer pig and the lowest Pi（0.003 27）was also found in Gaoligongshan pig.The total genetic rate unique determined by genetic variation and genetic unique that Diqing Tibetan pig（10.342 9）gave the highest contribution to the total genetic rate among Yunnan native pigs,the total genetic rate unique of both Gaoligongshan pig and Dahe pig were 1.059 4which provided the lowest contribution to Yunnan native pigs.【Conclusion】On the whole haplotype effects in terms of rich that Diqing Tibetan pig,Diannanxiaoer pig,Baoshan pig and Saba pig have the positive effect on the genetic diversity of Yunnan seven native pigs,Mingguang pig,Gaoligongshan pig and Dahe pig have the negative effect.

关 键 词：云南地方猪种 MTDNA D-LOOP区 遗传多样性 

分 类 号：S813.1[农业科学—畜牧学]