1α,25-(OH)_2D_3促进骨细胞形成及骨吸收活性（英文）  

Enhancement of Osteoclastogenesis and Bone Re-absorption by 1α,25-(OH)_2D_3 in Raw264.7 Cells in Vitro

作　　者：刘俊栋[1] 仝锡帅[2] 王东[2] 顾建红[2] 高倩[2] 刘宗平[2] 

机构地区：[1]江苏农牧科技职业学院,泰州225300 [2]扬州大学兽医学院,扬州225009

出　　处：《营养学报》2015年第6期607-610,613,共5页Acta Nutrimenta Sinica

基　　金：National Natural Science Foundation of China(grant nos.31172373 and 31372495);the National Science Foundation for Distinguished Young Scholars of China(grant no.31302154);the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(grant no.13KJB230002);the Project Funded by the Cultivate Middle-aged and Young Science Leaders of Colleges and Universities of Jiangsu Province(Su teachers [2010]27);the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions

摘　　要：目的研究1α,25-二羟维生素D_3(1α,25-(OH)_2D_3)对破骨细胞(osteoclast,OC)形成及骨吸收活性的影响。方法在巨噬细胞集落刺激因子(macrophage colony stimulating factor,M-CSF)和核因子-κB受体活化因子配体(receptor activator for nuclear factor-иB ligand,RANKL)诱导破骨细胞前体细胞RAW264.7细胞分化为OC的基础上,添加不同浓度1α,25-(OH)_2D_3(10^(-7),10^(-8)和10^(-9)mol/L),通过抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase,TRAP)染色鉴定成熟OC的形成,环境扫描电镜观察牛皮质骨片吸收陷窝。结果各浓度1α,25-(OH)_2D_3均可以促进RAW264.7细胞分化为OC,并增强其骨吸收活力。其中,10^(-8)mol/L的1α,25-(OH)_2D_3诱导效果最好,所形成的OC数最多,骨吸收活性最强。结论 1α,25-(OH)_2D_3能促进OC形成,增强骨吸收活性,从而调节骨代谢。Objective To study the effects of la,25-dihydroxyvitamin D3 （1α,25-（OH）2D3） on osteoclastogenesis and bone re-absorption activity. Methods RAW264.7 cells were treated with different concentrations of 1α,25-（OH）2D3 （10^-7, 10^-8 and 10^-9 mol/L） under the induction of macrophage-colony stimulating factor （M-CSF） and receptor activator of nuclear factor-κB ligand （RANKL） in vitro respectively. The formation of osteoclasts was detected by tartrate resistant acid phosphatase （TRAP） staining. Analysis of cortical sclerite lacunar re-absorption was carried out using a scanning electron microscope. Results The treatment of 1α,25-（OH）2D3 increased the number of osteoclasts and enhanced bone re-absorption activity. This effect was most obvious at the concentration of 108 mol/L. Conclusion 1α,25-（OH）2D3 could regulate bone metabolism by promoting osteoclastogenesis and bone re-absorption.

关 键 词：1α 25-(OH)_2D_3 破骨细胞 骨吸收 RAW264.7细胞 

分 类 号：R151[医药卫生—营养与食品卫生学]