杏仁核外源性nesfatin-1对大鼠探究行为的影响及机制  

Effects and mechanisms of exogenous nesfatin-1 injection in the central nucleus of the amygdale on explor- atory behavior in rats

作　　者：王巧玲[1] 徐珞[1] 

机构地区：[1]青岛大学医学院病理生理学教研室,青岛266021

出　　处：《中华行为医学与脑科学杂志》2015年第12期1066-1069,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：国家自然科学基金项目（31071014,81100260,81270460,81300281,81470815）;青岛市科技局项目（11-2-3-3-（2）-nsh;13-1-4-170-ich;14-2-3-3-nsh）

摘　　要：目的探讨中央杏仁核（CeA）微量注射nesfatin-1对大鼠探究行为的影响及机制。方法将50只大鼠随机分为5组：nesfatin-1低剂量（Lnesfatin-1）组、nesfatin-1高剂量（Hnesfatin-1）组、SHU9119组、SHU9119＋Lnesfatin-1组和对照组。采用CeA给药，观察大鼠在高架十字迷宫模型测试中探究行为的变化及焦虑状态下（10只大鼠）杏仁核nesfatin-1mRNA表达的改变。结果与对照组相比，Lnesfatin-1组和Hnesfatin—1组大鼠焦虑行为明显加强，表现为大鼠进入开放臂的次数显著降低（OE，t=4．16～2．87，P〈0．01），进人开放臂次数比例（OE％，t=2．39～4．39，P〈0．01～0．05）明显减少，在开放臂停留的时间比例（OT％，t=5．43～20．55，P〈0．01）明显下降，且呈量效依赖关系；与对照组相比，Lnesfatin-1组和Hnesfatin-1组大鼠焦虑程度显著增强，表现为大鼠向下探究次数明显减少（HD，t=6．97～16．73，P〈0．01），封闭臂后腿直立次数（RE，t=6．60～13．25，P〈0．01）明显增加，且呈量效依赖关系。如预先给予黑皮质素受体拮抗剂SHU9119可部分消除nesfatin-1的促焦虑作用（t=2．11～3．08，P〈0．01-0．05）。Real—timePCR结果显示，焦虑模型组大鼠nesfatin-1mRNA表达显著升高（t=3．40—5．58，P〈0．05）。结论杏仁核nesfatin-1可增加大鼠焦虑和恐惧感，该作用可能与黑皮质素通路有关。Objective To explore the effects and mechanisms of nesfatin-1 injection in the central nucle- us of the amygdale（CeA） on exploratory behavior in rats. Methods Fifty rats were divided into 5 groups random- ly ： nesfatin-1 low dose group, nesfatin-1 high dose group, SHU9119 group, SHU9119＋nesfatin-1 low dose group and control group.Drugs were administrated via CeA to examine behavioral changes of rats by elevated plus maze model test and nesfatin-1 mRNA expression in CeA of anxiety rats （ 10 rats）. Results Compared with the control group, anxiety behaviors of rats in nesfatin-1 low dose group and nesfatin-1 high dose group were improved remarkably, showing that the open arm entries were significantly reduced （OE, t= 4.16-12.87, P〈0.01 ）, open arm entry propor- tion was decreased remarkably （OE%, t= 2.39-4.39, P〈0.01-0.05）, and time proportion in open arm was de- creased significantly （OT%, t=5.43-20.55, P〈0.01） ,which presented dose dependent.Compared with the control group,anxiety degree of rats in nesfatin-1 low dose group and nesfatin-1 high dose group significantly enhanced, showing that the rats head dipping reduced obviously （HD, t= 6.97-16.73, P〈0.01） ,while rearing was increased significantly （ RE, t= 6.60-13.25, P〈0.01 ） ,which was presented dose dependent. Melanocortin receptor antagonist SHU9119 can partly eliminate nesfatin-1 induce-anxiety （ t = 2.11-3.08, P〈 0.01-0.05 ）. Real-time PCR results showed that nesfatin-1 mRNA expression of anxiety model rats was significantly increased （ t = 3.40-5.58, P〈 0.05 ）. Conclusion Amygdala nesfatin-1 can increase anxiety and fear of rats,which may be associated with mel- anocortin systems.

关 键 词：NESFATIN-1 焦虑 恐惧 高架十字迷宫 杏仁核 

分 类 号：R749.7[医药卫生—神经病学与精神病学]