姜黄素对人乳腺癌细胞T47D增殖与凋亡的影响及其机制  

Effect and mechanism of curcumin on the proliferation and apoptosis of human breast cancer cells T47D

作　　者：柳青 

机构地区：[1]曲靖市第一人民医院肿瘤科,云南曲靖655000

出　　处：《华夏医学》2015年第5期10-13,共4页Acta Medicinae Sinica

摘　　要：目的:探讨姜黄素对人乳腺癌细胞T47D增殖与凋亡的影响及机制。方法:不同浓度(20,40,80μmol/L)姜黄素作用于体外培养的人乳腺癌细胞T47D,采用MTT法分析细胞增殖抑制,流式细胞术分析细胞凋亡,RT-PCR检测Bcl-2mRNA表达。结果:姜黄素作用后,T47D细胞的生长抑制率明显上升,呈剂量和时间依赖性,细胞凋亡率增高,Bcl-2 mRNA表达水平降低。结论:姜黄素诱导T47D凋亡,抑制T47D生长,其机制可能与其下调Bcl-2mRNA表达有关。Objective：To investigate the effect of curcumin on the proliferation and apoptosis of T47D cells and its possible mechanism.Methods：Human breast cancer T47 Dcells were cultured and treated by curcumin in different concentrations（20,40 and 80μmol/L）in vitro.MTT assay was applied to determine the effect of curcumin on proliferation level of T47 Dcells,while apoptosis rate was assessed by flow cytometry.The mRNA level of Bcl-2 was measured by RT-PCR.Results：After curcumin treatment,MTT assay indicated that curcumin could inhibit the growth and proliferation of T47 Dcells in a time and concentration-dependent manner,and flow cytometry showed that apoptotic rate increased significantly.Furthermore,curcumin resulted in the obviously reduced expression of Bcl-2 mRNA in T47D cells.Conclusion：Curcumin can inhibit the growth of human breast cancer T47D cells and inducing apoptosis the mechanism may be related with the inhibition of Bcl-2mRNA expression.

关 键 词：姜黄素 乳腺癌 凋亡 BCL-2MRNA 

分 类 号：R737.9[医药卫生—肿瘤]