基于UPLC/LTQ-Orbitrap-MS的黄芩中黄酮碳苷的结构表征及同分异构体的区分  

Structural characterization of C-glycosyl flavonoids in Scutellaria baicalensis and differentiation of isomers by UPLC/LTQ-Orbitrap-MS

作　　者：谢彤[1] 徐建亚[1] 沈存思[1] 狄留庆[2] 汪受传[1] 单进军[1,2] 

机构地区：[1]南京中医药大学中医儿科学研究所,江苏省儿童呼吸疾病(中医药)重点实验室,江苏南京210023 [2]南京中医药大学药学院,江苏省中药高效给药系统工程技术研究中心,江苏南京210023

出　　处：《中草药》2015年第22期3318-3325,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金青年基金(81303295);国家自然科学基金面上项目(81373688);江苏省高校自然科学研究面上项目(13KJB360005)

摘　　要：目的建立黄芩Scutellaria baicalensis的UPLC/LTQ-Orbitrap-MS分析测定方法,研究黄芩中黄酮碳苷类成分,并区分其中的同分异构体。方法采集Orbitrap全扫描的质谱图,并触发动态数据依赖分析模式采集二级、三级和四级的碎片信息,通过图谱解析确定黄酮碳苷的结果,通过比对碎片信息区分同分异构体。结果在黄芩中共鉴定表征了19个黄酮碳苷类成分,并对其中的同分异构体进行区分。结论该方法能够准确地筛选黄芩中的黄酮碳苷类成分,并对同分异构体进行区分,为其他中药的成分分析提供参考。Objective An UPLC-LTQ-Orbitrap-MS spectrometry method has been developed for the efficient separation and detection of C-glycosyl flavonoids in the extract of Scutellaria baicalensis differentiation of isomers. Methods Data were acquired by Orbitrap. MS2, MS3, and MS4 data were triggered by data-dependent acquisition mode. Isomers of C-glycosyl flavonoids were distinguished by a comparison study of mass distribution. Results A total of 19 compounds were identified as C-glycosyl flavonoids, and several isomers were successfully discriminated. Conclusion This method is proved to be powerful for the identification of C-glycosyl flavonoids and provides the important complementary information for other flavonoid analysis.

关 键 词：黄芩 黄酮碳苷 UPLC/LTQ-Orbitrap-MS 同分异构体 结构表征 

分 类 号：R284[医药卫生—中药学]