基于支持向量机的5种大黄苷元治疗脑缺血的配伍研究  

Compatibility study of five rhubarb aglycones in treatment of cerebral ischemia based on support vector machine

作　　者：吴纯伟 宿爱山[1] 陈超[1,2,3] 梁生旺[1,2,3] 王淑美[1,2,3] 

机构地区：[1]广东药学院中药学院,广东广州510006 [2]广东高校中药质量工程技术研究中心,广东广州510006 [3]国家中医药管理局中药数字化质量评价技术重点研究室,广东广州510006

出　　处：《中草药》2015年第22期3377-3381,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81274060;81274059;81473413)

摘　　要：目的筛选大黄中5种能够治疗脑缺血的大黄苷元(芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚)的最佳配伍。方法采用均匀设计法将大鼠分为14组,每组15只。采用线栓法制备大鼠大脑中动脉闭塞(MCAO)模型,以神经功能症状评分、脑梗死面积为指标,探讨不同配伍的大黄苷元对脑缺血大鼠的影响,同时基于支持向量机(SVM)建立大黄苷元药效预测模型。结果经SVM回归分析,得到大黄苷元最优配伍为芦荟大黄素6.653 4 mg/kg、大黄酸26.000 8 mg/kg、大黄素11.004 2 mg/kg、大黄酚3.841 4 mg/kg和大黄素甲醚3.862 0 mg/kg。结论不同配比的大黄苷元能有效改善大鼠脑缺血的各个指标,采用均匀设计结合SVM的模拟预测方法优选出了5种大黄苷元组分的最佳配比。Objective To optimize the compatibility of five rhubarb aglycones（aloe-emodin, rhein, emodin, rhubarb, and physcion） in treatment of cerebral ischemia. Methods The rats were divided into 14 groups based on uniform design. The rats were subjected to right middle cerebral artery occlusion（MCAO） with the suture-occluded method by Longa. The effect of rhubarb aglycone was evaluated using neurological symptoms and infarction area percentage as indexes. The support vector（SVM） machine model was established for pharmacodynamics prediction of rhubarb aglycone. Results The model established in this study could predict the drug actions of different combinations. The best compatibility was aloe-emodin 6.653 4 mg/kg, rhein 26.000 8 mg/kg, emodin 11.004 2 mg/kg, chrysophanol 3.841 4 mg/kg, and physcion 3.862 0 mg/kg. Conclusion Different compatibilities of rhubarb aglycone are effective in the treatment of ischemia to rats. Uniform design combined with SVM method can be more efficient to realize the dose optimization for rhubarb aglycone prescription.

关 键 词：大黄苷元 配伍 脑缺血 支持向量机 均匀设计 芦荟大黄素 大黄酸 大黄素 大黄酚 大黄素甲醚 

分 类 号：R285.5[医药卫生—中药学]