p53、p73与生存素及caspase-7蛋白酶在喉癌组织中的表达及临床意义  

Expression and its clinical significance of p53,p73,survivin and caspase-7 in laryngeal carcinoma

作　　者：凌航[1] 廖前进[1] 钟外生[1] 崔捷[1] 王季轩 陈杰[1] 

机构地区：[1]中南大学湘雅医学院附属肿瘤医院湖南省肿瘤医院头颈外一科,湖南长沙410013

出　　处：《中国耳鼻咽喉颅底外科杂志》2015年第6期447-452,共6页Chinese Journal of Otorhinolaryngology-skull Base Surgery

基　　金：湖南省科学技术厅(03SSY310)

摘　　要：目的探讨p53、p73、生存素(survivin)及caspase7蛋白酶在喉癌组织中的表达及其临床意义。方法采用免疫组化(SP法)检测49例喉癌组织和42例喉正常组织中突变型p53、p73、survivin及caspase-7蛋白的表达水平,并分析其与喉癌临床病理参数和预后的关系。结果突变型p53、p73、survivin及caspase-7蛋白在喉癌组织中有较高阳性表达率,分别为71.4%、87.8%、91.8%、95.9%,而在喉正常组织中其阳性表达率明显降低(P<0.05),分别为21.4%、23.8%、76.2%、47.6%。四种蛋白表达与喉癌临床病理参数和预后分析显示,突变型p53、p73蛋白表达随着喉癌临床分期的演进而增加,且喉癌中有淋巴结转移的p53、p73蛋白表达显著高于无淋巴结转移组(P<0.05),p73蛋白表达还与喉癌病理分级和临床不良预后密切相关(P<0.05);survivin蛋白表达与喉癌临床分期和病理分级相关(P<0.05);caspase-7蛋白高表达患者,其病理分级和预后较好(P<0.05)。相关性分析发现喉癌组织中Survivin蛋白表达分别与p73(r=0.372,P<0.01)、caspase-7(r=0.476,P<0.01)蛋白表达呈正相关。结论突变型p53、p73、survivin及caspase-7蛋白在喉癌组织中均存在高表达,Survivin蛋白表达分别与p73、caspase-7蛋白表达正相关,p73、survivin、caspase-7蛋白表达均与患者临床预后有关,可成为喉癌治疗、预后判断的潜在生物学指标。Objective To investigate the expression of p53, p73, survivin and caspase-7 in laryngeal carcinoma and its relationships with the clinical characteristics of this tumor. Methods Immunohistochemical staining （ SP method） was used for detecting the expression of p53, p73, survivin and caspase-7 in 49 specimens of laryngeal carcinoma and 42 of normal laryngeal mucosa. Results The positive expression rates of p53, p73, survivin and easpase-7 in the specimens of laryngeal carcinoma were 71.4% , 87.8% , 91.8% and 95.9% respectively, which were significantly higher than those in the normal laryngeal mucosa tissue（ 21.4% , 23.8% , 76.2% and 47.6% respectively, all P 〈 0.05 ）. The correlation analysis between these four factors and clinieopathological characteristics as well as prognosis of the tumor showed that the positive expression rates of p53, p73 were positively correlated with clinical stages, and the positive expression rates in specimens with lymph node metastasis were significantly higher than those without （ both P 〈 O. 05 ）. The correlation analysis also revealed that the expression of p73 was closely correlated with tumor＇ s pathological grade and patients＇ overall survival （ P 〈 0.05 ） , survivin with clinical stage and pathological grade （ P 〈 0.05 ） , Caspase-7 with tumor differentiation and overall survival （ P 〈 0.05 ）. The expression of survivin was positively correlated with which of p73 and caspase-7 （r =0.372, 0. 476, both P 〈 0. O1 ）. Conclusions The expression of p53, p73, survivin and caspase-7 in laryngeal carcinoma is higher than that in normal laryngeal mucosa. The expression of survivin is positively correlated with which of p73, caspase-7. The expression of p73, survivin, caspase-7 is relevant to patients＇ prognosis, which means they may be potential targets of therapy and biological indicators of prognosis in laryngeal carcinoma.

关 键 词：喉癌 突变型P53蛋白 P73蛋白 survivin蛋白 caspase-7蛋白酶 

分 类 号：R739.65[医药卫生—肿瘤]