几种抑制剂抗晚期糖基化/脂质过氧化终产物(AGEs/ALEs)作用的比较  

Inhibitory Effect of Different Inhibitor Formation of Advanced Glycation Endproducts(AGEs)and Advanced Lipoxidation Endproducts(ALEs) in Vitro

作　　者：刘荟萃[1] 李巨秀[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《中国食品学报》2015年第10期11-18,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31101217)

摘　　要：晚期糖基化终产物(Advanced glycation endproducts,AGEs)和脂质过氧化终产物(Advanced lipoxidation endproducts,ALEs)与糖尿病及衰老有关疾病的发病机理密切相关。为比较维生素类(吡哆胺、硫胺素)、黄酮类(表儿茶素、槲皮素、芦丁)和酚酸类(阿魏酸)对AGEs/ALEs的抑制特性,建立了牛血清蛋白-果糖(BSA-fructose)、牛血清蛋白-丙酮醛(BSA-MGO)、脂肪酸(油酸、亚油酸)-氨基酸(赖氨酸、精氨酸)模拟反应体系,同时比较了上述3类抑制剂对丙酮醛(MGO)的清除能力。结果表明,除硫胺素对BSA-fructose模拟反应体系中AGEs无抑制作用外,其他抑制剂对BSA-fructose、BSA-MGO、脂肪酸-氨基酸模拟体系中的AGEs和ALEs均有抑制作用,并有浓度-剂量依赖关系。吡哆胺对BSA-fructose、BSA-MGO模拟体系中的AGEs的抑制作用显著(P<0.05),强于其他参试抑制剂,对ALEs的抑制作用最差;3种黄酮对AGEs/ALEs的抑制作用都较强,其中表儿茶素对AGEs的抑制作用是黄酮类中最强的,槲皮素对ALEs的抑制作用是黄酮类中最强的。阿魏酸对ALEs的抑制效果优于对AGEs。3类抑制剂均可有效清除MGO,随着反应时间延长,对MGO清除率最低的硫胺素也可达到79.96%。Advanced glycation endproducts（AGEs） and advanced lipoxidation endproducts（ALEs） are closely related to many human diseases such as diabetes and age-related diseases. In order to investigate inhibitory effect of vitamins（pyridoxamine, thiamine）, flavonoids（epicatechin, quercetin, rutin） and phenolic acid（ferulic acid） on formation of AGEs/ALEs, BSA-fructose, BSA-MGO and fatty acid（oleic acid, linoleic acid）-amino acid（lysine, arginine） modle systems were carried out. Moreover, the ability of inhibitors to scavenge MGO was also studied. Besides thiamine have no inhibitory effect on formation of AGEs in BSA-fructose modle system, all experimental inhibitors could effectively inhibit the formation of AGEs/ALEs and the inhibition ratio had dose-dependent effect with concentration. The inhibition rate of pyridoxamine to AGEs was significant（P〈0.05） higher than others, but to ALEs was lowest; The flavonoids exhibited very strong inhibitory activity against the formation of AGEs/ALEs, the inhibitory effect of epicatechin on AGEs was the strongest in flavonoids, the inhibitory effect of quercetin on ALEs was the strongest in flavonoids; The inhibition rate of ferulic acid to ALEs greater than AGEs. All inhibitors could effectively scavenge MGO, the lowest scavenging rate was79.96%, which obtained by thiamine.

关 键 词：抑制剂 晚期糖基化终产物 晚期脂质过氧化产物 抑制特性 比较 

分 类 号：R96[医药卫生—药理学]