脂肪细胞分化成熟过程中瘦素对Hedgehog信号通路的上调作用  

Up-regulating effect of leptin on Hedgehog signaling pathway in the process of adipocyte differentiation and maturity

作　　者：刘亚莉[1] 孟华[2] 迟素敏[1] 

机构地区：[1]第四军医大学基础部生理学教研室,陕西西安710032 [2]第四军医大学西京医院军人特诊中心,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2015年第12期1602-1605,1610,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(31200876;31371412)

摘　　要：目的研究瘦素(leptin)对Hedgehog(Hh)信号通路的调节作用。方法使用3T3-L1细胞诱导脂肪细胞分化,实时定量PCR和Western blot法分别检测脂肪细胞分化过程中Hh信号通路关键转录因子胶质瘤相关癌基因同源物(Gli)mRNA和蛋白的表达,以及leptin对其表达水平的影响。结果在脂肪细胞分化过程中,诱导后的0、4、7、10 d,Gli1、Gli2、Gli3的mRNA和蛋白的表达逐渐受到抑制,以Gli1最为显著;而应用100 ng/m L leptin则可以促进Gli1、Gli2、Gli3的mRNA和蛋白表达。结论在脂肪细胞分化成熟过程中,leptin和Hh具有交互作用,leptin可上调Hh通路信号分子Gli的表达,leptin可能通过激活Hh信号通路发挥抑制脂肪生成作用。Objective To study the regulating effect of leptin on Hedgehog（ Hh） signaling pathway. Methods 3T3-L1 cells were used to induce adipocyte differentiation. Real-time quantitative PCR and Western blotting were performed to detect the mRNA and protein levels of Gli,the key molecule in Hh signaling pathway,and investigate the effect of leptin on the levels in the process of adipocyte differentiation. Results At 0,4,7,10 days of adipose differentiation,mRNA and protein expressions of Gli1,Gli2 and Gli3 in Hh signaling pathway were gradually restrained and the down-regulation of Gli1 mRNA was the most significant. However,100 ng / m L leptin promoted the mRNA and protein expressions of Gli1,Gli2 and Gli3 during adipose differentiation. Conclusion In the process of adipocyte differentiation and maturity,there is an interaction between leptin and Hh signaling pathway. Leptin can upregulate the expression of Gli in Hh pathway,and leptin may play a role in the inhibition of adipogenesis through activating Hh signaling pathway.

关 键 词：脂肪细胞 分化 瘦素(leptin) HEDGEHOG信号通路 GLI 

分 类 号：Q459[生物学—生理学] R392.12[医药卫生—免疫学]