外周血淋巴细胞染色体标本制片室内质量控制  

Peripheral blood lymphocyte chromosome specimen tablet internal quality control

作　　者：赖专华[1] 武永平[1] 路建超[1] 高涛[1] 

机构地区：[1]宝鸡市疾病预防控制中心,陕西721006

出　　处：《医学动物防制》2016年第1期112-114,共3页Journal of Medical Pest Control

基　　金：宝鸡市卫生局科研立项课题(2014-58)

摘　　要：目的了解外周血淋巴细胞染色体标本制片的影响因素,寻找出最佳的室内质量控制制作条件。方法比较制片过程中不同的接种血量、培养温度、低渗时间以及离心速度对外周血中染色体标本制作的影响情况。结果外周血染色体制片过程中最佳的室内质量控制条件是接种血量0.7×32规格注射器针头20~25滴;培养温度应该严格控制在37℃±0.5℃;最佳低渗时间是15~20 min;最佳离心速度1 500 r/min^2 000 r/min;固定液应在使用前临时配置,放置1 h应废弃;玻片冷藏处理的效果比热水处理后的好。结论外周血淋巴细胞染色体标本制片的室内质量控制条件有接种血量、培养温度和低渗时间等,相关实验室应予关注,对辐射防护作出正确的评价。Objective To understand the factors of the production of peripheral blood lymphocyte chromosome aberration specimen,find out the optimal production conditions of internal quality control. Methods Comparison of the production process of different inoculation volume,culture temperature,low infiltration time and centrifugal speed in peripheral blood Chromosome aberration specimen making influence situation,looking for the best production effect of internal quality control. Results In the peripheral blood chromosome aberration producers outside the laboratory process the best internal quality control conditions are inoculated volume is0. 7 × 32 syringe needle 20-25 drops; culture temperature should be strictly controlled at 37 ℃ ± 0. 5 ℃;best low infiltration time is 6-9 minutes; best centrifugal speed of 1 500 r / min-2 000 r / min; and the fixed liquid should be temporary configuration before use,place 1 hours should be abandoned; slide storage treatment effect is better than hot water treatment. Conclusions Peripheral blood lymphocyte chromosome specimen producer inoculation of indoor quality control conditions of HP,culture temperature and low permeability,etc.relevant laboratory should be attention,to correctly evaluate the radiation protection.

关 键 词：外周血 淋巴细胞 染色体 质量控制 

分 类 号：R394[医药卫生—医学遗传学]