金黄色葡萄球菌对中性粒细胞粘弹性的影响  

Effects of Staphylococcus Aureus on Viscoelastic Propertion of Rat Polymorphonuclear Leukocyte

作　　者：宋关斌[1] 刘保安[1] 李新平[1] 秦建[1] 蔡绍皙[1] 

机构地区：[1]重庆大学生物工程学院,重庆400044

出　　处：《重庆大学学报（自然科学版）》2002年第3期75-77,共3页Journal of Chongqing University

基　　金：国家自然科学基金资助项目 (19972 0 77)

摘　　要：采用微管吸吮技术研究了中性粒细胞在外源病原微生物金黄色葡萄球菌刺激而免疫激活状态下的粘弹特性及不同的细菌浓度对中性粒细胞粘弹性的影响。同时以标准线性固体模型对实验数据进行了拟合。结果表明 :中性粒细胞免疫激活后的粘弹性参数K1、K2 、μ与对照组比较均显著增高 ,但随着细菌浓度的提高而表现出饱和效应 ;用金黄色葡萄球菌分泌物 (培养液 )处理中性粒细胞后 。A micropipette aspiration technique was adopted to investigate the viscoelasticity of rat polymorphonuclear leukocyte (PMN) after treated with vary concentration staphylococcus aureus and its secretions. A standard linear solid model (kelvin model) was used to fit the experimental data. The results show that the viscoelastic parameters K 1?K 2 and μ of PMNs are higher than that of control after stimulated by staphylococcus aureus. Further, they showed a staturated phenomenon after the concentration of staphylococcus aureus increased. But K 1?K 2 and μ of PMNs have no remarkable difference compared with control after treated with suspend solution of staphylococcus arueus.

关 键 词：金黄色葡萄球 中性粒细胞 粘弹性 微管吸吮技术 临床治疗 炎症 

分 类 号：R364.5[医药卫生—病理学]