Ti-6Al-7Nb喷砂酸蚀处理后表面特性和生物相容性的实验研究  

Experimental Study on the Ti-6Al-7Nb Surface Properties and Biological Compatibility by Sandblast and Acid-etching

作　　者：孙鸿婉 李志刚[1] 张静莹[2] 

机构地区：[1]辽宁医学院附属第二医院口腔科,辽宁锦州121000 [2]大连大学医学院口腔科,辽宁大连116622

出　　处：《中国医科大学学报》2016年第1期12-16,30,共6页Journal of China Medical University

基　　金：国家自然科学基金(51371042);中国博士后自然科学基金(2014M551097);大连市科技计划(2013E11SF057);辽宁省博士科研启动基金(20141198)

摘　　要：目的通过机械打磨、喷砂酸蚀2种不同方法分别处理纯钛(Ti)和Ti-6Al-7Nb(TC20)表面,在2种钛片表面接种大鼠成骨细胞,探讨钛片表面的机械性能和大鼠成骨细胞的黏附、增殖情况。方法将实验分为4组:Ti机械打磨组、Ti喷砂酸蚀组、TC20机械打磨组、TC20喷砂酸蚀组,在4组钛片上接种大鼠成骨细胞,使用扫描电镜和能谱分析仪分析4组钛片表面形貌和元素含量,用3D激光共聚焦显微镜分析4组钛片表面的粗糙度,电感耦合等离子发色光谱仪分析钛片内部元素析出量,倒置显微镜观察大鼠成骨细胞形貌,MTT测量大鼠成骨细胞增殖情况。结果 Ti和TC20机械打磨组表面呈机械划痕,Ti和TC20喷砂酸蚀组表面拥有大量孔洞形貌;喷砂酸蚀处理的Ti和TC20表面有大量成骨细胞附着,经统计学分析成骨细胞在喷砂酸蚀组表面增殖能力强于机械打磨组,且Ti-6Al-7Nb喷砂酸蚀组强于Ti喷砂酸蚀组(P<0.05)。结论喷砂酸蚀处理的TC20表现出良好的机械性能和生物相容性,更能促进骨结合。Objective To process pure titanium （Ti） and Ti- 6Al- 7Nb （TC20） surface by mechanical grinding and sandblast and acid-etching （SLA）, discuss the mechanical properties of the titanium surface, and evaluate the adhesion and proliferation of rat osteoblasts on the two kinds of titanium surface. Methods The experiment was divided into 4 groups： Ti mechanical grinding group, Ti SLA group, TC20 mechanical grinding group, and TC20 SLA group. Then osteoblasts were seeded onto the titanium plate of different groups. Titanium surface morphology and elemental composition were analyzed by SEM and EDX. Surface roughness of titanium plate was analyze 3D laser confocal microscopy. The internal elements of titanium and titanium alloy precipitation were measured by inductively coupled plasma spectrometer. Adhesion of osteoblast cells on different titanium surface were observed by scanning electron microscope, and the proliferation of osteoblast was analyzed by MTT test. Results Scratches were observed on Ti and TC20 surface in mechanical grinding group, a large hole morphology was found in SLA groups. There were a lot of rat osteoblasts adhered to the titanium surface with SLA processing, and the proliferative ability of osteoblasts in SLA groups was stronger than mechanical grinding greups through the statistical analysis （ P 〈 0.05 ）. Conclusion TC20 with SLA processing showed better mechanical properties and biocompatibility, which can significantly promote bone union.

关 键 词：TI Ti-6Al-7Nb 喷砂酸蚀 成骨细胞 

分 类 号：R783.1[医药卫生—口腔医学]