丝素蛋白/羟基磷灰石支架对成骨诱导脂肪干细胞活性及成骨性能的影响  

Effect of silk fibroin/hydroxyapatite scaffold on the viability and osteogenic properties of adipose-derived stem cells under osteogenic induction

作　　者：刘浩[1] 褚亚伟[1] 丁涛[1] 程力[1] 朱浩明[1] 

机构地区：[1]南京医科大学附属无锡市人民医院,江苏省无锡市214023

出　　处：《中国组织工程研究》2015年第45期7224-7229,共6页Chinese Journal of Tissue Engineering Research

基　　金：无锡市医院管理中心医学技术重大项目(YGZX1111)~~

摘　　要：背景:成骨诱导后的脂肪干细胞与可降解丝素蛋白/羟基磷灰石支架复合,可望研制出一种具有良好生物相容性及成骨性能的新型骨融合材料。目的:探讨丝素蛋白/羟基磷灰石支架对成骨诱导脂肪干细胞增殖活性及成骨性能的影响。方法:获取大鼠脂肪干细胞后体外贴壁培养、扩增,将第3代细胞用条件培养液进行成骨方向的定向诱导培养、扩增,然后接种到预湿的丝素蛋白/羟基磷灰石材料上作为实验组,以相同条件下置入盖玻片和脂肪干细胞培养作为对照组。倒置相差显微镜观察细胞在材料中的生长情况,MTT法检测材料对细胞增殖活性的影响,碱性磷酸酶活性测定评价其成骨能力。结果与结论:成骨诱导后的脂肪干细胞在丝素蛋白/羟基磷灰石材料上能够良好地黏附和增殖。实验组和对照组细胞增殖活性及碱性磷酸酶活性比较,差异无显著性意义(P>0.05),证实脂肪干细胞活性及成骨性能不受材料影响,说明丝素蛋白/羟基磷灰石复合材料具有良好的细胞相容性。BACKGROUND： Adipose-derived stem cells under osteogenic induction can be combined with biodegradable silk fibroin/hydroxyapatite scaffold, which is expected to develop a new biocompatible and osteogenic bone fusion material.OBJECTIVE： To study the effect of silk fibroin/hydroxyapatite composite on the viability and osteogenic properties of adipose-derived stem cells after osteogenic induction. METHODS： Adipose-derived stem cells were obtained from rat＇s fat tissue, then adherently cultured, proliferated and passaged in vitro. Passage 3 cells were cultured in conditioned medium for osteogenic induction, and then seeded onto silk fibroin/hydroxyapatite scaffold as experimental group. Adipose-derived stem cells cultured on the cover glasses at the same condition acted as control group. The cellular morphology, proliferation and differentiation were assessed respectively by means of phase contrast microscope, MTT assay and alkaline phosphatase activity measurement. RESULTS AND CONCLUSION： After osteogenic induction, adipose-derived stem cells could adhere to the scaffold material and proliferate on the surface of silk fibroin/hydroxyapatite scaffold normally. No significant difference was found in cell proliferation and alkaline phosphatase activity between the experimental and control groups（P〉 0.05）, suggesting the cellular activity and function were not affected by the material. These findings indicate that silk fibroin/hydroxyapatite composite material has good cytocompatibility.

关 键 词：丝素蛋白 羟基磷灰石类 干细胞 脂肪组织 生物相容性材料 组织工程 脂肪干细胞 羟基磷灰石 生物相容性 支架材料 成骨性 复合型支架 

分 类 号：R394.2[医药卫生—医学遗传学]