人参皂苷诱导骨髓间充质干细胞促进创伤性颅脑损伤的神经再生  

Ginsenosides-induced bone marrow mesenchymal stem cells promote nerve regeneration in traumatic brain injury

作　　者：覃军[1] 陈家康[1] 李学东[1] 麦用军[1] 肖振勇[1] 

机构地区：[1]广西医科大学第四附属医院(广西柳州市工人医院),广西壮族自治区柳州市545000

出　　处：《中国组织工程研究》2015年第45期7292-7297,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:以往研究表明骨髓间充质干细胞治疗神经疾病方面已经取得了一定成效,能明显促进神经功能改建,但关于基因及药物调控的相关研究目前尚未取得突破性进展。目的:探讨人参皂苷诱导骨髓间充质干细胞分化对创伤性颅脑损伤后神经再生的影响。方法:采用液压冲击法建立大鼠颅脑创伤模型,随机分为颅脑损伤组、骨髓间充质干细胞组、人参皂苷诱导分化的骨髓间充质干细胞组。移植后2周采用Western blot检测创伤脑组织神经生长因子及脑源性神经营养因子表达水平,免疫组织化学法检测BrdU标记的阳性细胞数量;移植后1,3 d及1,2周进行动物神经学缺损评分。结果与结论:人参皂苷诱导分化的骨髓间充质干细胞组大鼠脑创伤组织神经生长因子及脑源性神经营养因子蛋白表达水平明显高于骨髓间充质干细胞组和颅脑损伤组(P<0.05);人参皂苷诱导分化的骨髓间充质干细胞组Brd U标记的阳性细胞数量明显多于骨髓间充质干细胞组和颅脑损伤组(P<0.05);移植后3 d及1,2周,人参皂苷诱导分化的骨髓间充质干细胞组大鼠神经功能障碍评分低于骨髓间充质干细胞组和颅脑损伤组(P<0.05)。结果表明经人参皂苷诱导分化后的骨髓间充质干细胞移植可以促进创伤性颅脑损伤大鼠的神经再生,较单独应用骨髓间充质干细胞移植效果显著。BACKGROUND： Previous studies have shown that bone marrow mesenchymal stem cells in the treatment of neurological diseases have achieved some success, which can promote neurological alterations; however, there is no breakthrough on gene and drug regulation.OBJECTIVE： To investigate the influence of ginsenosides-induced differentiation of bone marrow mesenchymal stem cells on nerve regeneration after traumatic brain injury. METHODS： A traumatic brain injury model was built in rats using hydraulic shock method, and then rat models were randomly divided into model group（traumatic brain injury group）, bone marrow mesenchymal stem cell group, ginsenosides group（ginsenosides induced differentiation of bone marrow mesenchymal stem cells）. At 2 weeks after transplantation, western blot assay was used to detect protein expression levels of nerve growth factor and brain-derived neurotrophic factor, immunohistochemistry assay used to detect the number of BrdU-positive cells. At 1, 3 days and 1, 2 weeks after transplantation, modified neurological severity scores were recorded. RESULTS AND CONCLUSION： The expression levels of nerve growth factor and brain-derived neurotrophic factor protein were significantly higher in the ginsenosides group than the bone marrow mesenchymal stem cell group and model group（P〈0.05）. The number of Brd U positive nerve cells was also higher in the ginsenosidesgroup than the bone marrow mesenchymal stem cell group and model group（P〈0.05）. At 3 days and 1, 2 weeks after transplantation, the modified neurological severity scores in the ginsenosides group were lower than those in the bone marrow mesenchymal stem cell group and model group（P〈0.05）. These findings indicate that ginsenoside-induced bone marrow mesenchymal stem cell transplantation can promote nerve regeneration in rats with traumatic brain injury, which has better outcomes than bone marrow mesenchymal stem cell transplantation alone.

关 键 词：颅脑损伤 人参皂甙类 骨髓 间质干细胞移植 组织工程 干细胞 移植 人参皂苷 骨髓间充质干细胞 大鼠 神经再生 神经生长因子 脑源性神经营养因子 

分 类 号：R394.2[医药卫生—医学遗传学]