两种食品防腐剂与牛血清白蛋白相互作用  

The Interaction Between Natamycin/nisin and Bovine Serum Albumin

作　　者：李田[1,2] 程正军[1,2] 曹丽君[1,2] 蒋晓慧[1,2] 

机构地区：[1]西华师范大学化学化工学院,四川南充637009 [2]西华师范大学四川省化学合成与污染控制重点实验室,四川南充637009

出　　处：《西华师范大学学报（自然科学版）》2015年第4期360-366,共7页Journal of China West Normal University(Natural Sciences)

基　　金：四川省教育厅重点项目(12ZA171)

摘　　要：运用多光谱技术研究纳他霉素/乳酸链球菌素(natamycin/nisin)与牛血清白蛋白(BSA)间的相互作用.结果表明:natamycin和nisin与BSA间的猝灭机理分别为静态猝灭和联合猝灭.natamycin/nisin绑定BSA位点I从而导致BSA结构变化.此外,计算了这两个绑定反应的结合常数、结合位点数和结合距离.The interaction of natamycin/nisin with bovine serum albumin （BSA） has been investigated by multiple spectroscopic techniques. The results indicated that the quenching mechanism between BSA and natamycin or nisin was a static quenching or combined quenching （ static and dynamic） process. Natamycin/nisin was primarily bound to site I （subdomain IIA） of BSA, which led to its structural changes. In addition, the binding constants, binding site numbers and binding distance of the two systems were also calculated.

关 键 词：牛血清白蛋白 纳他霉素 乳酸链球菌素 荧光光谱 结合常数 

分 类 号：O65[理学—分析化学]