二烯丙基二硫对视网膜母细胞瘤Y79细胞生长、侵袭的影响及机制探讨  

Effects of diallyl disulfide on proliferation and invasion of retinoblastoma cells Y79 and the mechanism

作　　者：刘越峰[1] 罗卫民[1] 张勇[1] 钟晓东[1] 

机构地区：[1]湖北医药学院附属十堰市太和医院,湖北十堰442000

出　　处：《山东医药》2015年第47期33-35,共3页Shandong Medical Journal

基　　金：湖北省教育厅科学研究计划指导性项目(B2015477);十堰市科学技术研究与开发项目计划(14Y40)

摘　　要：目的观察二烯丙基二硫(DADS)对视网膜母细胞瘤Y79细胞生长、侵袭的影响,并探讨其机制。方法培养人视网膜母细胞瘤Y79细胞,分为miRNA阴性对照组(转染40μmol/L scramble)、miR-222M组(转染40μmol/L miR-222-mimics)、DADS阴性对照组(加入10μmol/L DMSO)、DADS组(加入200μmol/L DADS)、DADS+miR-222I组(转染40μmol/L miR-200b-inhibitors和200μmol/L DADS)。采用qRT-PCR法检测不同浓度DADS处理的Y79细胞中的miR-222,采用MTT法和Transwell侵袭实验分别检测各组细胞增殖和侵袭情况。结果 0、25、50、100、200和400μmol/L不同浓度的DADS处理的Y79细胞中的miR-222相对表达量分别为2.823±0.250、2.343±0.226、1.955±0.267、1.567±0.096、0.875±0.189、0.718±0.126。miRNA阴性对照组、miR-222M组、DADS阴性对照组、DADS组、DADS+miR-222I组Y79细胞OD570值分别为0.727±0.167、0.949±0.070、0.712±0.178、0.497±0.126、0.351±0.102,Transwell穿膜细胞数分别为128±8、179±16、127±12、76±8、45±14,miR-222M组、DADS组、DADS+miR-222I组分别与miRNA阴性对照组、DADS阴性对照组比较,DADS组与DADS+miR-222I组比较,P均<0.05。结论 DADS通过下调miR-222的表达抑制视网膜母细胞瘤Y79细胞的生长与侵袭。Objective To investigate the effects of diallyl disulfide（ DADS） on proliferation and invasion of retinoblastoma cells Y79 and to investigate the mechanism. Methods The retinoblastoma cells Y79 were cultured and divided into the miRNA negative control group（ tansfected by 40 μmol / L scramble）,miR-222 group（ tansfected by 40 μmol / L miR-222-mimics）,DADS negative control group（ added with 10 μmol / L DMSO） and DADS group（ added with 200μmol /L DADS） and DADS ＋ miR-222 I group（ transfected by 40 μmol /L miR-200b-inhibitors ＋ 200 μmol /L DADS）.The expression of miR-222 treated by different concentrations of DADS in Y79 cells was detected by qRT-PCR. The proliferation and invasion of retinoblastoma cells Y79 in vitro were determined with MTT and Transwell invasion assay. Results The miR-222 expression of Y79 cells treated with 0,25,50,100,200 and 400 μmol / L DADS was 2. 823 ± 0. 250,2. 343 ± 0. 226,1. 955 ± 0. 267,1. 567 ± 0. 096,0. 875 ± 0. 189 and 0. 718 ± 0. 126,respectively. The OD570 of miR-222 was respectively 0. 727 ± 0. 167,0. 949 ± 0. 070,0. 712 ± 0. 178,0. 497 ± 0. 126 and 0. 351 ± 0. 102 in the miRNA negative control group,miR-222 M group,DADS negative control group,DADS group and DADS ＋ miR-222 I group. Transwell invasion assay results showed that the number of cells permeating the matrigel significantly decreased to 128 ± 8,179 ± 16,127 ± 12,76 ± 8 and 45 ± 14. Significant difference was respectively found between the miR-222 M group,DADS group,DADS ＋ miR-222 I group and the miRNA negative control group and DADS negative control group（ all P〈0. 05）. Conclusion DADS can inhibit the proliferation and invasion of retinoblastoma cells Y79 by down-regulating the expression of miR-222.

关 键 词：视网膜母细胞瘤 微小RNA 222 二烯丙基二硫 细胞增殖 细胞侵袭 

分 类 号：R739.7[医药卫生—肿瘤]